DISTRIBUTION OF ESTROGEN RECEPTOR-IMMUNOREACTIVE CELLS IN MONKEY HYPOTHALAMUS - RELATIONSHIP TO NEURONS CONTAINING LUTEINIZING-HORMONE-RELEASING HORMONE AND TYROSINE-HYDROXYLASE
DISTRIBUTION OF ESTROGEN RECEPTOR-IMMUNOREACTIVE CELLS IN MONKEY HYPOTHALAMUS - RELATIONSHIP TO NEURONS CONTAINING LUTEINIZING-HORMONE-RELEASING HORMONE AND TYROSINE-HYDROXYLASE
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DOI：
10.1159/000126810
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发表时间：
1995-01-01
影响因子：
4.1
通讯作者：
LERANTH, C
中科院分区：
文献类型：
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作者：
HERBISON, AE;HORVATH, TL;LERANTH, C
性腺类固醇影响控制垂体激素分泌的神经内分泌细胞活性的确切位置和机制尚不清楚。本研究确定了猴下丘脑中雌激素受体（ER）免疫反应细胞的分布，并检测了ER是否通过黄体生成素释放激素（LHRH）和/或含多巴胺的神经元表达。在切除卵巢（n = 2）和雌激素加孕激素治疗（n = 2）的食蟹猕猴和单个切除卵巢的非洲绿猴中，测定了er免疫反应细胞的分布。在所有猴子视前区、终纹床核、脑室周围区、腹内侧核和弓形核中检测到大量对内质网具有免疫反应的细胞，而与类固醇状态无关。室旁核有少量er免疫反应细胞，视上核未见。5只猴子的双标记实验显示，432个LHRH神经元中没有一个具有可检测到的ER免疫反应性。在A11、A12和A14细胞组中发现了酪氨酸羟化酶（TH）染色的神经元，尽管A11和A12神经元混杂在ER免疫反应细胞中并与ER免疫反应细胞相邻，但1,652个TH免疫反应细胞中没有一个含有ER免疫反应。这些结果表明，猕猴下丘脑中er免疫反应细胞的分布方式与其他哺乳动物相似，尽管并非所有含有孕激素受体（pr）的大脑区域都被发现表达er。双标记实验进一步证明LHRH神经元不具有内质网，并表明，与其他物种一样，雌激素对灵长类LHRH神经元的影响是间接的和/或非基因组性的。与大鼠和绵羊不同，猴子下丘脑多巴胺能神经元中没有发现内质网免疫反应的证据。在特定的下丘脑核以及多巴胺能神经元中，内质网和报道的PR受体定位的差异提出了在灵长类下丘脑中并非所有含有PR的细胞都表达内质网的可能性。
The precise sites and mechanisms by which gonadal steroids influence the activity of neuroendocrine cells controlling pituitary hormone secretion are poorly understood. The present study has determined the distribution of estrogen receptor (ER)-immunoreactive cells in the monkey hypothalamus and examined whether ERs are expressed by luteinising hormone releasing hormone (LHRH)-and/or dopamine-containing neurones. The distribution of ER-immunoreactive cells was determined in ovariectomised (n = 2) and estrogen plus progesterone-treated (n = 2) cynomolgus macaques and in a single ovariectomised African green monkey. Large numbers of cells immunoreactive for the ER were detected in the preoptic area, bed nucleus of the stria terminalis, periventricular area and ventromedial and arcuate nuclei of all monkeys irrespective of the steroid status. Smaller numbers of ER-immunoreactive cells were found in the paraventricular, but not supraoptic nucleus. Double-labeling experiments in sections from ah 5 monkeys revealed that none of the 432 LHRH neurons examined possessed detectable ER immunoreactivity. Neurones stained for tyrosine hydroxylase (TH) were identified in the A11, A12, and A14 cell groups and, although A11 and A12 neurones were intermingled amongst and found adjacent to ER-immunoreactive cells, none of the 1,652 TH-immunoreactive cells examined contained ER immunoreactivity. These results show that ER-immunoreactive cells in the monkey hypothalamus are distributed in a manner similar to that observed in other mammalian species although not all brain regions reported to contain progesterone receptors (PRs) in these species of monkey were found to express ERs. The double-labelling experiments provide further evidence that LHRH neurones do not possess ERs and indicate that, as in other species, estrogen influences on primate LHRH neurones are indirect and/or non-genomic in nature. Unlike the rat and sheep, no evidence was found for ER immunoreactivity in hypothalamic dopaminergic neurones of the monkey. The discrepancy in ER and reported PR receptor localisation within specific hypothalamic nuclei as well as in dopaminergic neurones raises the possibility that not all PR-containing cells may express ERs in the primate hypothalamus.