Synthesis of Structurally Defined Cationic Polythiophenes for DNA Binding and Gene Delivery.
Synthesis of Structurally Defined Cationic Polythiophenes for DNA Binding and Gene Delivery.
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DOI：
10.1021/acsami.7b17948
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发表时间：
2018-01
影响因子：
9.5
通讯作者：
Chi Zhang;Jinkai Ji;Xiaoyan Shi;Xiaoyu Zheng;Xuewei Wang;F. Feng
中科院分区：
文献类型：
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作者：
Chi Zhang;Jinkai Ji;Xiaoyan Shi;Xiaoyu Zheng;Xuewei Wang;F. Feng
水溶性共轭聚合物在生物成像、疾病诊断和治疗等领域具有广阔的应用前景。然而，将具有可控性和区域规则性的WCP用于生物应用的报道很少。在本工作中，我们通过Kumada催化转移缩聚（KCTP）合成了含酯侧链的聚噻吩（P3ET），并证实了准“活”链增长机制。此外，我们通过氨解不同链长的P3ET得到了阳离子区域规整聚噻吩（cPTs），并研究了其与DNA的结合能力和基因传递性能。得益于细胞内活性氧（ROS）的光控产生，阳离子聚噻吩成功地将DNA递送到肿瘤细胞中，而无需额外的聚合物物质。
Water-soluble conjugated polymers (WCPs) have prospective applications in the field of bioimaging, disease diagnosis, and therapy. However, the use of WCPs with controllability and regioregularity for bioapplications have scarcely been reported. In this work, we synthesized polythiophenes containing ester side chains (P3ET) via Kumada catalyst-transfer polycondensation (KCTP) and confirmed a quasi-"living" chain-growth mechanism. In addition, we obtained cationic regioregular polythiophenes (cPTs) by aminolysis of P3ET with varied chain lengths, and studied DNA binding capability and gene delivery performance. Benefiting from photocontrolled generation of intracellular reactive oxygen species (ROS), the cationic polythiophenes successfully delivered DNA into tumor cells without additional polymer species.