LOCALIZATION OF A TOXOPLASMA-GONDII RHOPTRY PROTEIN BY IMMUNOELECTRON MICROSCOPY DURING AND AFTER HOST-CELL PENETRATION
LOCALIZATION OF A TOXOPLASMA-GONDII RHOPTRY PROTEIN BY IMMUNOELECTRON MICROSCOPY DURING AND AFTER HOST-CELL PENETRATION
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DOI：
10.1111/j.1550-7408.1992.tb04844.x
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发表时间：
1992-07-01
期刊：
影响因子：
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通讯作者：
SCHWARTZMAN, JD
中科院分区：
文献类型：
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作者：
SAFFER, LD;MERCEREAUPUIJALON, O;SCHWARTZMAN, JD
我们免疫定位弓形虫棒状体蛋白（ROP 1）之前和之后的寄生虫宿主细胞侵入人成纤维细胞和TG 180小鼠肉瘤细胞的电子显微镜和免疫金标记使用单克隆抗体（Tg 49）或单特异性兔抗血清（α-249）。 在寄生虫生长的所有阶段，ROP 1都在体内被发现，但很少在棒状体的梗中，即使在那些看起来空的梗中也是如此。 在宿主细胞入侵后，ROP 1立即与寄生虫空泡膜结合。 在入侵后数小时内，在寄生虫空泡膜上可检测到的ROP 1的量显著减少。 ROP 1的定位表明在宿主细胞中感染的早期建立中的作用，与先前的工作一致，该工作表明ROP 1的单克隆抗体（包括在这些研究中使用的单克隆抗体）干扰渗透增强现象。
We immunolocalized a Toxoplasma gondii rhoptry protein (ROP1) before and after parasite host cell invasion of human fibroblasts and TG180 murine sarcoma cells by electron microscopy and immunogold labeling using either a monoclonal antibody (Tg49) or a monospecific rabbit antiserum (alpha-249). At all stages of parasite growth ROP1 was found within the body but rarely within the peduncle of rhoptries, even in those that appeared empty. Immediately after host cell invasion ROP1 was associated with the parasitophorous vacuole membrane. Within hours after invasion the amount of ROP1 immunodetectable on the parasitophorous vacuole membrane was markedly decreased. The localization of ROP1 suggests a role in the early establishment of infection in host cells, consistent with previous work that has indicated that monoclonal antibodies to ROP1 (including the one used in these studies) interfere with the phenomenon of penetration enhancement.