Comparison of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK2 system for identification of Acinetobacter clinical isolates
Comparison of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK2 system for identification of Acinetobacter clinical isolates
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DOI：
10.1016/j.diagmicrobio.2013.07.013
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Moon, Dae-Soo
中科院分区：
文献类型：
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作者：
Lee, Min Jung;Jang, Sook Jin;Moon, Dae-Soo
由于准确的菌种鉴定是正确治疗不动杆菌感染的必要条件，我们对167株临床分离不动杆菌进行了4种鉴定方法的性能比较，以确定最佳鉴定方法。为了获得更多的非鲍曼不动杆菌（NBA）菌株作为目标菌株，我们首先在使用VITEK 2系统鉴定的495株临床不动杆菌中鉴定出鲍曼不动杆菌。由于495株鲍曼不动杆菌中有371株经gyrB多重PCR和bla(OXA51-like) PCR鉴定为鲍曼不动杆菌，我们对其余124株NBA菌株和52株鲍曼不动杆菌进行了rpoB基因测序和16S rRNA基因测序。对于种水平的不动杆菌鉴定，rpoB基因测序、16S rRNA基因测序、gyrB多重PCR和VITEK 2的准确率分别为98.2%、93.4%、77.2%和35.9%。gyrB多重PCR对于ACB复合体的检测非常有用，因为它与最终鉴定的ACB复合体菌株的一致性率为100%。rpoB基因测序和16S rRNA基因测序可用于不动杆菌的鉴定。(C) 2014爱思唯尔公司版权所有。
Since accurate identification of species is necessary for proper treatment of Acinetobacter infections, we compared the performances of 4 bacterial identification methods using 167 Acinetobacter clinical isolates to identify the best identification method. To secure more non-baumannii Acinetobacter (NBA) strains as target strains, we first identified Acinetobacter baumannii in a total of 495 Acinetobacter clinical isolates identified using the VITEK 2 system. Because 371 of 495 strains were identified as A. baumannii using gyrB multiplex 1 PCR and bla(OXA51-like) PCR, we performed rpoB gene sequencing and 16S rRNA gene sequencing on remaining 124 strains belonging to NBA and 52 strains of A. baumannii. For identification of Acinetobacter at the species level, the accuracy rates of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK 2 were 98.2%, 93.4%, 77.2%, and 35.9%, respectively. The gyrB multiplex PCR seems to be very useful for the detection of ACB complex because its concordance rates to the final identification of strains of ACB complex were 100%. Both the rpoB gene sequencing and the 16S rRNA gene sequencing may be useful in identifying Acinetobacter. (C) 2014 Elsevier Inc. All rights reserved.