Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line
Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line
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DOI：
10.1016/j.jep.2003.09.048
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发表时间：
2004-01-01
影响因子：
5.4
通讯作者：
Neungton, N
中科院分区：
文献类型：
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作者：
Moongkarndi, P;Kosem, N;Neungton, N
本研究以人乳腺癌(SKBR3)细胞为模型系统，研究了芒果果皮粗甲醇提取物(CME)的抗增殖、抗凋亡和抗氧化作用。不同浓度(0~50µg/ml)的CME作用于SKBR3细胞48h后，用四甲基偶氮唑蓝(3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑(MTT)比色法测定细胞存活率。CME对乳腺癌细胞的增殖抑制作用呈剂量依赖性，ED50为9.25+/-0.64lg/ml。通过细胞形态变化和寡核体DNA片段的检测，我们发现CME对乳腺癌细胞的抑制作用与细胞凋亡有关。此外，不同浓度和孵育时间的CME对ROS的产生也有抑制作用。这些研究表明，芒果果皮的甲醇提取物具有很强的抗增殖、抗氧化和诱导细胞凋亡的作用。由此可见，该物质具有不同的活性，对癌症的化学预防具有剂量依赖性和暴露时间依赖性。(C)2003爱思唯尔爱尔兰有限公司。保留所有权利。
This study was designed to determine the antiproliferative, apoptotic and antioxidative properties of crude methanolic extract (CME) from the pericarp of Garcinia mangostana (family Guttiferae) using human breast cancer (SKBR3) cell line as a model system. SKBR3 cells were cultured in the presence of CME at various concentrations (0-50 mug/ml) for 48 h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyl tetrazolium bromide (MTT) assay. CME showed a dose-dependent inhibition of cell proliferation with ED50 of 9.25 +/- 0.64 Lg/ml. We found that antiproliferative effect of CME was associated with apoptosis on breast cancer cell line by determinations of morphological changes and oligonucleosomal DNA fragments. In addition, CME at various concentrations and incubation times were also found to inhibit ROS production. These investigations suggested that the methanolic extract from the pericarp, of Garcinia mangostana had strong antiproliferation, potent antioxidation and induction of apoptosis. Thus, it indicates that this substance can show different activities and has potential for cancer chemoprevention which were dose dependent as well as exposure time dependent. (C) 2003 Elsevier Ireland Ltd. All rights reserved.