Number and function of endothelial progenitor cells as a marker of severity for diabetic vasculopathy
Number and function of endothelial progenitor cells as a marker of severity for diabetic vasculopathy
复制标题
DOI：
10.1161/01.atv.0000237750.44469.88
复制
发表时间：
2006-09-01
影响因子：
8.7
通讯作者：
Avogaro, Angelo
中科院分区：
文献类型：
--
作者：
Fadini, Gian Paolo;Sartore, Saverio;Avogaro, Angelo
目的 - 外周动脉疾病（PAD）是糖尿病的一种威胁性并发症。由于内皮祖细胞（EPC）参与新血管生成和血管稳态的维持，因此它们的损伤可能在糖尿病血管病变的发病机制中发挥作用。本研究旨在确定 2 型糖尿病患者中 EPC 的数量和功能是否与 PAD 严重程度相关。方法和结果 - EPC 通过 CD34、CD133 和 KDR 的表达来定义，并通过流式细胞术对 127 名患有和不患有 PAD 的糖尿病患者进行定量。 PAD 严重程度根据颈动脉粥样硬化和腿部动脉粥样硬化闭塞症的临床阶段进行评估。与非 PAD 患者相比，患有 PAD 的糖尿病患者循环 EPC 显着减少 53%，并且 EPC 水平与颈动脉狭窄程度和腿部跛行阶段呈负相关。此外，患有 PAD 的糖尿病患者与未患有 PAD 的糖尿病患者相比，培养的 EPC 的克隆形成和粘附能力显着降低。 结论 - 这项研究表明，EPC 减少与 PAD 严重程度相关，并且患有 PAD 的糖尿病受试者中 EPC 功能发生改变，强化了 EPC 失调在糖尿病血管病变中的发病作用。 EPC 计数可能被认为是糖尿病外周动脉粥样硬化的新生物标志物。
Objective - Peripheral arterial disease (PAD) is a threatening complication of diabetes. As endothelial progenitor cells (EPCs) are involved in neovasculogenesis and maintenance of vascular homeostasis, their impairment may have a role in the pathogenesis of diabetic vasculopathy. This study aimed to establish whether number and function of EPCs correlate with PAD severity in type 2 diabetic patients.Methods and Results - EPCs were defined by the expression of CD34, CD133 and KDR, and quantified by flow cytometry in 127 diabetic patients with and without PAD. PAD severity has been assessed as carotid atherosclerosis and clinical stage of leg atherosclerosis obliterans. Diabetic patients with PAD displayed a significant 53% reduction in circulating EPCs versus non-PAD patients, and EPC levels were negatively correlated with the degree of carotid stenosis and the stage of leg claudication. Moreover, the clonogenic and adhesion capacity of cultured EPCs were significantly lower in diabetic patients with PAD versus patients without.Conclusions - This study demonstrates that EPC decrease is related to PAD severity and that EPC function is altered in diabetic subjects with PAD, strengthening the pathogenetic role of EPC dysregulation in diabetic vasculopathy. EPC count may be considered a novel biological marker of peripheral atherosclerosis in diabetes.