Screening of rice resources against rice black-streaked dwarf virus and mapping of resistant QTL.
Screening of rice resources against rice black-streaked dwarf virus and mapping of resistant QTL.
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DOI：
10.3724/sp.j.1006.2010.01258
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Wan, J. M.
中科院分区：
文献类型：
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作者：
Wang BaoXiang, Wang BaoXiang;Jiang Ling, Jiang Ling;Wan, J. M.
水稻黑条矮缩病毒(RBSDV)在江苏、浙江两省流行。为了筛选抗病种质和发现新的抗病基因/QTL，我们在大田条件下对311份粳稻品种进行了抗病鉴定。结果表明，没有一个品种对RBSDV免疫。江苏省目前种植的24个主栽品种的发病率在10.0%~29.0%之间，江苏推广的287个品种中71.5%的品种发病率在10.0%~30.0%之间。利用感病品种贵潮2号与抗病品种Koshihikari杂交的162个重组自交系进行了数量性状基因座(QTL)分析。用自然侵染法对RBSDV的抗性进行评分。在位于第3染色体上的标记RM7和RM5748之间定位了1个控制RBSDV抗性的QTL(QRBSDV3)，该QTL解释了总表型变异的17.1%，LOD评分为5.4。这一正向抗性效应来自Koshihikari。进一步的分析表明，含有qRBSDV3等位基因的品系对RBSDV的抗性显著增强。研究结果对利用分子标记辅助选择技术选育抗病新品种具有重要意义。
Rice black-streaked dwarf virus (RBSDV) disease become epidemic in Jiangsu and Zhejiang provinces. To screen resistant germplasms and discover new resistance genes/QTLs against RBSDV, we evaluated the resistance to RBSDV in 311 japonica varieties in field test. The results showed that no variety was immune to RBSDV. The disease rate of 24 main japonica varieties grown in Jiangsu province at present was 10.0%-29.0% and that of 71.5% varieties in 287 japonica varieties popularized in Jiangsu before was between 10.0% and 30.0%. Furthermore, quantitative trait loci (QTL) analysis was conducted by using 162 recombinant inbred lines (RILs), which derived from a cross between Guichao 2, a susceptible indica variety, and Koshihikari, a japonica variety with resistance to RBSDV. RBSDV resistances were evaluated using natural infection methods by scoring the disease rating. One putative QTL (qRBSDV3) controlling RBSDV resistance was mapped between the marker RM7 and RM5748 on chromosome 3, which explained 17.1% of the total phenotypic variation with LOD score of 5.4. The positive resistant effect contributed from Koshihikari. Further analysis revealed that the lines harboring the alleles of qRBSDV3 exhibited significantly increased resistance to RBSDV. The results should be very useful for breeding new RBSDV-resistant cultivars by marker-assisted selection (MAS).