PROLONGATION OF THE NORMAL LIFESPAN AND INHIBITION OF SPONTANEOUS CANCER BY ANTIOXIDANTS
PROLONGATION OF THE NORMAL LIFESPAN AND INHIBITION OF SPONTANEOUS CANCER BY ANTIOXIDANTS
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DOI：
10.1093/geronj/16.3.247
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发表时间：
1961-01-01
期刊：
影响因子：
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通讯作者：
HARMAN, D
中科院分区：
文献类型：
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作者：
HARMAN, D
材料与方法AKR(雄性)、C3H(雌性)和Swiss(雄性)均由缅因州巴尔港杰克逊纪念实验室在断奶后即刻获得。他们得到了临时的食物。一种造粒的小鼠食物，在造粒前加入(除对照外)4种还原剂中的1种：1)半胱氨酸盐酸盐，2)2-巯基乙胺盐酸盐，3)2，2‘-二氨基二乙基二硫化物二盐酸盐和4)羟胺盐酸盐；前3种化合物以1%w的程度添加，最后一种以1%w和2%w添加。每月测定每笼小鼠的平均体重。在C3H和瑞士小鼠的情况下，肿瘤的数量也被记录下来；没有看到肉眼肿瘤。我们要感谢利兰·肖特博士和查尔斯·里格斯博士在小鼠护理方面的帮助，以及哈丁·B·琼斯博士和约翰·H·劳伦斯博士对在加州大学伯克利分校唐纳实验室进行的漫长实验的支持。
MATERIALS AND METHODS AKR (males), C3H (females), and Swiss (males) were obtained from the Jackson Memorial Laboratory of Bar Harbor, Maine, immediately after weaning. They were fed ad lib. a pelleted mouse food to which, before pelleting, had been added (with the exception of the controls) 1 of 4 reducing agents: 1) cysteine hydrochloride, 2) 2-mercaptoethylamine hydrochloride, 3) 2, 2'-diaminodiethyl disulfide dihydrochloride, and 4) hydroxylamine hydrochloride; the first 3 compounds were added to the extent of 1% w, while the last was added at 1% w and 2% w. The pelleted foods were prepared in batches at intervals of 1 to 2 months. The average weight of the mice in each cage was determined monthly. In the case of the C3H and Swiss mice, the number of tumors were also recored; gross tumors were not seenWe should like to acknowledge our indebtedness to Dr. Leland Short and Dr. Charles Riggs for their assistance in the care of the mice and to Dr. Hardin B. Jones and Dr. John H. Lawrence for their support of the lengthy experiment conducted at the Donner Laboratory of the University of California, Berkeley, California.