Collaborative environmental DNA sampling from petal surfaces of flowering cherry Cerasus?×?yedoensis ‘Somei-yoshino’ across the Japanese archipelago
Collaborative environmental DNA sampling from petal surfaces of flowering cherry Cerasus?×?yedoensis ‘Somei-yoshino’ across the Japanese archipelago
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对日本列岛樱花花瓣表面的环境 DNA 进行协作采样
DOI：
10.1007/s10265-018-1017-x
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发表时间：
2018
影响因子：
2.8
通讯作者：
Haruo Suzuki
中科院分区：
文献类型：
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作者：
Tazro Ohta;Takeshi Kawashima;Natsuko O. Shinozaki;Akito Dobashi;◯Satoshi Hiraoka;Tatsuhiko Hoshino;Keiichi Kanno;Takafumi Kataoka;Shuichi Kawashima;Motomu Matsui;Wataru Nemoto;Suguru Nishijima;Natsuki Suganuma;Haruo Suzuki
最近的研究表明，环境DNA几乎随处可见。花瓣表面是一种很有吸引力的组织，用于研究环境DNA在自然界中的扩散，因为它们与外部环境隔离，直到花蕾开放，只有这样，花瓣表面才能积累环境DNA。在这里，我们进行了一个众包实验，即“Ohanami Project”，以获取2015年春季日本群岛各地樱花‘Somei-Yoshino’花瓣表面的环境DNA样本。C.×yedoenssis是日本最受欢迎的园林樱桃品种，该品种的无性系每年春天都会同时开花。数据收集覆盖了几乎每个县，总共收集了149名合作者的577个DNA样本。初步的扩增序列分析表明，环境DNA迅速附着在花瓣表面。值得注意的是，我们在广泛分布的样本中发现了其他常见植物物种的DNA；这些DNA可能来自日本雪松的花粉。我们的分析支持了我们的信念，即花瓣开花后的表面是揭示自然界环境DNA动态的一个有前途的目标。我们实验的成功也表明，众包环境DNA分析在生态学研究中具有相当大的价值。
Recent studies have shown that environmental DNA is found almost everywhere. Flower petal surfaces are an attractive tissue to use for investigation of the dispersal of environmental DNA in nature as they are isolated from the external environment until the bud opens and only then can the petal surface accumulate environmental DNA. Here, we performed a crowdsourced experiment, the “Ohanami Project”, to obtain environmental DNA samples from petal surfaces ofCerasus×yedoensis‘Somei-yoshino’ across the Japanese archipelago during spring 2015.C. ×yedoensisis the most popular garden cherry species in Japan and clones of this cultivar bloom simultaneously every spring. Data collection spanned almost every prefecture and totaled 577 DNA samples from 149 collaborators. Preliminary amplicon-sequencing analysis showed the rapid attachment of environmental DNA onto the petal surfaces. Notably, we found DNA of other common plant species in samples obtained from a wide distribution; this DNA likely originated from the pollen of the Japanese cedar. Our analysis supports our belief that petal surfaces after blossoming are a promising target to reveal the dynamics of environmental DNA in nature. The success of our experiment also shows that crowdsourced environmental DNA analyses have considerable value in ecological studies.