Identification of new Xlsirt family members in the Xenopus laevis oocyte.
Identification of new Xlsirt family members in the Xenopus laevis oocyte.
复制标题
非洲爪蟾卵母细胞中 Xlsirt 家族新成员的鉴定。
DOI：
10.1016/s0925-4773(02)00459-8
复制
发表时间：
2003
影响因子：
2.6
通讯作者：
Etkin,LaurenceD
中科院分区：
文献类型：
--
作者：
Zearfoss,NRuth;Chan,AgnesP;Kloc,Malgorzata;Allen,LuettaH;Etkin,LaurenceD
非洲爪哇短穿插重复转录本(Xlsirts)是一个非编码RNA家族，由作为植物定位元件的特定重复序列的存在定义。以前的研究表明，Xlsirts作为定位元件对RNA进行定位，并在植物皮层锚定mRNA。然而，Xlsirts的身份尚不清楚，这些Xlsirts包含出现在大脑皮层的家庭成员。我们从一个卵母细胞cDNA文库中鉴定出17个新的Xlsirt cDNAs。除了与非编码序列相关外，重复序列也存在于具有开放阅读框架的cDNA中。Xlsirt RNA在正确的方向上具有重复，能够定位到植物皮质。我们的观察表明，大脑皮质存在一个不同种类的Xlsirt RNAs群体，这个群体既包含非编码RNAs，也包含编码蛋白的RNAs，这些RNAs可能在胚胎的后续发育中发挥重要作用。
Xenopus laevis short interspersed repeat transcripts (Xlsirts) are a family of noncoding RNAs defined by the presence of a specific repeated sequence that acts as a vegetal localization element. Previous studies have demonstrated that Xlsirts function as localization elements to localize RNA and also in anchoring mRNA at the vegetal cortex. However, the identity of the Xlsirts containing family members present at the cortex was unknown. We identified 17 new Xlsirt cDNAs from an oocyte cDNA library. In addition to being associated with noncoding sequences, the repeats were also present in cDNAs with open reading frames. Xlsirt RNAs with repeats in the correct orientation were capable of localizing to the vegetal cortex. Our observations demonstrate that a heterogeneous population of Xlsirt RNAs is present at the cortex and that this population contains both noncoding RNAs and RNAs encoding proteins that are likely to play important roles in the subsequent development of the embryo.