Enhanced antioxidant effect of caffeic acid phenethyl ester and Trolox in combination against radiation induced-oxidative stress
Enhanced antioxidant effect of caffeic acid phenethyl ester and Trolox in combination against radiation induced-oxidative stress
复制标题
咖啡酸苯乙酯和维生素E联合使用增强抗辐射诱导氧化应激的抗氧化作用
DOI：
10.1016/j.cbi.2013.10.022
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发表时间：
2014-01-25
影响因子：
5.1
通讯作者：
Hai, Chun-Xu
中科院分区：
文献类型：
--
作者：
Bai, Hua;Liu, Rui;Hai, Chun-Xu
抗氧化剂的组合被认为比单一的抗氧化剂更有效，因为当抗氧化剂组合在一起时，它们相互协同支持，产生放大的效果。发现生物活性成分之间的增强作用或协同作用对于抵抗氧化应激和改善健康益处是有价值的。本研究旨在探讨天然抗氧化剂咖啡酸苯乙酯(CAPE)与合成抗氧化剂Trolox在L929细胞自由基化学生成、微粒体脂过氧化和辐射氧化损伤模型体系中的协同作用。基于CAPE和Trolox之间的分子间相互作用，本研究表明CAPE和Trolox在清除三种不同的自由基和抑制三种不同体系引发的脂质过氧化方面具有协同作用。CAPE和Trolox同时加入L929细胞，通过减少ROS的产生，保护质膜，增加还原型谷胱甘肽/氧化谷胱甘肽的比值，以及核因子红系P45相关因子2(NRF2)介导的关键抗氧化酶的表达，增强了对辐射所致氧化损伤的预防作用。我们的结果首次表明CAPE和Trolox联合应用可能具有协同的抗氧化作用，进一步表明CAPE和Trolox的联合作用主要是通过直接清除ROS，抑制脂质过氧化，促进Nrf2调节的谷胱甘肽过氧化物酶和谷胱甘肽还原酶的表达来促进GSH的氧化还原循环。(C)2013爱思唯尔爱尔兰有限公司。保留所有权利。
Combinations of antioxidants are believed to be more effective than single antioxidant because when antioxidants are combined they support each other synergistically to create a magnified effect. Discovering the enhancer effects or synergies between bioactive components is valuable for resisting oxidative stress and improving health benefits. The aim of this study was to investigate a possible cooperation of natural antioxidant caffeic acid phenethyl ester (CAPE) with synthetic antioxidant Trolox in the model systems of chemical generation of free radicals, lipid peroxidation of microsomes and radiation-induced oxidative injury in L929 cells. Based on the intermolecular interaction between CAPE and Trolox, the present study shows a synergistic effect of CAPE and Trolox in combination on elimination of three different free radicals and inhibition of lipid peroxidation initiated by three different systems. CAPE and Trolox added simultaneously to the L929 cells exerted an enhanced preventive effect on the oxidative injury induced by radiation through decreasing ROS generation, protecting plasma membrane and increasing the ratios of reduced glutathione/oxidized glutathione and the expression of key antioxidant enzymes mediated by nuclear factor erythroid 2 p45-related factor 2 (Nrf2). Our results showed for the first time that administration of CAPE and Trolox in combination may exert synergistic antioxidant effects, and further indicate that CAPE and Trolox combination functions mainly through scavenging ROS directly, inhibiting lipid peroxidation and promoting redox cycle of GSH mediated by Nrf2-regulated glutathione peroxidase and glutathione reductase expression. (C) 2013 Elsevier Ireland Ltd. All rights reserved.