Increased expression of luteinizing hormone/human chorionic gonadotropin receptor gene in human endometrial carcinomas.
Increased expression of luteinizing hormone/human chorionic gonadotropin receptor gene in human endometrial carcinomas.
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DOI：
10.1210/jcem.79.5.7962347
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发表时间：
1994-11
期刊：
影响因子：
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通讯作者：
J. Lin;Z M Lei;S. Lojun;C V Rao;P. Satyaswaroop;T G Day
中科院分区：
文献类型：
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作者：
J. Lin;Z M Lei;S. Lojun;C V Rao;P. Satyaswaroop;T G Day
正常人子宫内膜表达LH/hCG受体基因。在本研究中，我们调查是否人子宫内膜癌也表达这种受体基因。逆转录巢式聚合酶链反应扩增了人子宫内膜癌的LH/hCG受体序列，就像从正常人子宫内膜和作为阳性对照组织的人卵巢中扩增的那样。北方印迹显示，子宫内膜癌含有大量的LH/hCG受体转录本，并随着肿瘤分级的增加而增加。Western免疫印迹显示，所有级别的子宫内膜癌都含有多种免疫反应性受体蛋白，其丰度高于正常子宫内膜。原位杂交和免疫细胞化学显示，不仅存在，但也较高的LH/hCG受体信使核糖核酸和受体蛋白水平的子宫内膜癌腺体相比，腺体在正常子宫内膜。配体印迹表明，35千道尔顿的蛋白受体可以结合[125 I]hCG，这种结合被过量的未标记的hCG抑制。子宫内膜癌中的结合率高于正常子宫内膜。子宫内膜癌样本的萎缩和子宫颈内腺体含有很少或没有受体。总之，我们的研究结果表明，人类子宫内膜癌不仅包含，而且似乎过表达LH/hCG受体相比，正常子宫内膜。这一新的发现介绍了以前未知的可能性，LH及其受体在人类子宫内膜癌中的作用。
Normal human endometrium expresses LH/hCG receptor gene. In the present study, we investigated whether human endometrial carcinomas also express this receptor gene. Reverse transcription-nested polymerase chain reaction amplified LH/hCG receptor sequences from human endometrial carcinoma just as it did those from normal human endometrium and human ovary as a positive control tissue. Northern blotting demonstrated that endometrial carcinomas contain a greater abundance of multiple LH/hCG receptor transcripts, which increased with increasing tumor grade. Western immunoblotting revealed that all grades of endometrial carcinomas contain multiple immunoreactive receptor proteins in greater abundance than normal endometrium. In situ hybridization and immunocytochemistry demonstrated not only the presence, but also higher LH/hCG receptor messenger ribonucleic acid and receptor protein levels in glands of endometrial carcinoma compared to glands in normal endometrium. Ligand blotting demonstrated that the 35-kilodalton protein receptor could bind [125I]hCG and that this binding was inhibited by excess unlabeled hCG. The binding was higher in endometrial carcinoma than in normal endometrium. Atrophic and endocervical glands from endometrial carcinoma samples contained very few or no receptors. In summary, our results demonstrate that human endometrial carcinomas not only contain but also appear to overexpress LH/hCG receptors compared to normal endometrium. This novel finding introduces previously unsuspected possibilities concerning the role of LH and its receptors in human endometrial carcinomas.