The Biological Activity of H.Pylori SlyD in Vitro
The Biological Activity of H.Pylori SlyD in Vitro
复制标题
H.Pylori SlyD的体外生物学活性
DOI：
10.1111/hel.12057
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发表时间：
2013-10-01
期刊：
影响因子：
4.4
通讯作者：
Yuan Yuan
中科院分区：
文献类型：
--
作者：
Kang Dan;Gong Yuehua;Yuan Yuan
目的：研究幽门螺杆菌的体外生物活性。方法：在大肠杆菌（E. coli）中构建幽门螺杆菌（h.p ylori） sld原核表达载体，采用固定化金属亲和层析法纯化重组sld （rsld）。通过CCK-8、细胞周期、caspase-3活性、基质侵袭试验和双层软琼脂集落形成效率检测rsld对AGS细胞的增殖、凋亡、侵袭、转化作用。western blot和免疫荧光法检测PCNA、KI-67、caspase-3、MMP-9的表达。结果：CCK-8检测结果显示，与AGS或AGS + PBS组相比，AGS + rslld组细胞增殖呈时间和剂量依赖性增加(p
Aim: To investigate the biological activity of the H. pylori SlyD in vitro.Methods: Helicobacter pylori (H. pylori) slyD prokaryotic expression vector was carried out in Escherichia coli (E. coli), and recombination SlyD (rSlyD) was purified by immobilized metal affinity chromatography. The proliferation, apoptosis, invasion, transformation effects of rSlyD on AGS cells was detected by CCK-8, cell cycle, caspase-3 activity, matrigel invasion assay, and double-deck soft agar colony forming efficiency. In addition, the expressions of PCNA, KI-67, caspase-3, and MMP-9 were detected by western blot and immunofluorescence assay, respectively.Results: The CCK-8 assay revealed that cell proliferation was increased in a time and dose-dependent manner in AGS + rSlyD group compared with that of AGS or AGS + PBS group (p