Generation of HIV Latency in Humanized BLT Mice
Generation of HIV Latency in Humanized BLT Mice
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DOI：
10.1128/jvi.06120-11
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发表时间：
2012-01-01
影响因子：
5.4
通讯作者：
Garcia, J. Victor
中科院分区：
文献类型：
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作者：
Denton, Paul W.;Olesen, Rikke;Garcia, J. Victor
在此，我们证明替诺福韦、恩曲他滨和拉替拉韦的组合可有效抑制人源化 BLT 小鼠的外周和全身 HIV 复制。我们还证明，经过抗逆转录病毒疗法（ART）治疗的人源化 BLT 小鼠体内含有潜伏感染的静息人类 CD4（+）T 细胞，这些 T 细胞可以在体外诱导产生 HIV。我们观察到，BLT 小鼠中受感染的静息人类 CD4(+) T 细胞水平在接受抑制性 ART 患者中观察到的循环水平范围内。这些结果证明了人源化 BLT 小鼠作为测试新型 HIV 根除策略的体内功效的有吸引力的模型的潜力。
Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir effectively suppresses peripheral and systemic HIV replication in humanized BLT mice. We also demonstrate that antiretroviral therapy (ART)-treated humanized BLT mice harbor latently infected resting human CD4(+) T cells that can be induced ex vivo to produce HIV. We observed that the levels of infected resting human CD4(+) T cells present in BLT mice are within the range of those observed circulating in patients undergoing suppressive ART. These results demonstrate the potential of humanized BLT mice as an attractive model for testing the in vivo efficacy of novel HIV eradication strategies.