Direct Comparison of Therapeutic Effects on Diabetic Polyneuropathy between Transplantation of Dental Pulp Stem Cells and Administration of Dental Pulp Stem Cell-Secreted Factors.
Direct Comparison of Therapeutic Effects on Diabetic Polyneuropathy between Transplantation of Dental Pulp Stem Cells and Administration of Dental Pulp Stem Cell-Secreted Factors.
复制标题
DOI：
10.3390/ijms21176064
复制
发表时间：
2020-08-23
影响因子：
5.6
通讯作者：
Naruse K
中科院分区：
文献类型：
--
作者：
Kanada S;Makino E;Nakamura N;Miyabe M;Ito M;Hata M;Yamauchi T;Sawada N;Kondo S;Saiki T;Minato T;Miyazawa K;Goto S;Matsubara T;Naruse K
干细胞移植是治疗糖尿病多发性神经病的一种新的治疗方法。牙髓干细胞（DPSC）是有吸引力的干细胞来源，因为DPSC可以从拔牙中分离并冷冻保存，同时保持活力。在这项研究中，我们直接比较了DPSC移植和DPSC分泌因子（DPSC-SFs）对糖尿病多发性神经病的疗效。注射链脲佐菌素8周后，将DPSC（1.0 × 106个细胞/只）或DPSC-SFs（1.0 mL/只）注入糖尿病SD大鼠单侧后肢骨骼肌。DPSC移植和DPSC-SF给药不影响糖尿病大鼠的血糖水平和体重。DPSC移植和DPSC-SF给药均显著改善糖尿病大鼠坐骨神经传导速度和坐骨神经血流量，并伴有肌束大小、骨骼肌血管密度和表皮内神经纤维密度的增加，而DPSC和DPSC-SF的结果之间无差异。这些结果表明，DPSC移植和DPSC-SF给药在移植/给药后4周对糖尿病多发性神经病的疗效主要是由于在移植/给药的早期阶段从移植的DPSC或直接注射的DPSC-SF分泌的多个分泌物。
Stem cell transplantation is a potential novel therapy for diabetic polyneuropathy. Dental pulp stem cells (DPSCs) are attractive stem cell sources because DPSCs can be isolated from extracted teeth and cryopreserved while retaining viability. In this study, we directly compared the efficacy of the transplantation of DPSCs and the administration of the secreted factors from DPSCs (DPSC-SFs) on diabetic polyneuropathy. Eight weeks after streptozotocin injection, DPSCs (1.0 × 106 cells/rat) or DPSC-SFs (1.0 mL/rat) were administered into the unilateral hindlimb skeletal muscles of diabetic Sprague–Dawley rats. DPSC transplantation and DPSC-SF administration did not affect blood glucose levels and body weights in the diabetic rats. Both DPSC transplantation and DPSC-SF administration significantly ameliorated sciatic nerve conduction velocity and sciatic nerve blood flow, accompanied by increases in muscle bundle size, vascular density in the skeletal muscles and intraepidermal nerve fiber density in the diabetic rats, while there was no difference between the results for DPSCs and DPSC-SFs. These results suggest that the efficacy of both DPSC transplantation and DPSC-SF administration for diabetic polyneuropathy four weeks after transplantation/administration was mainly due to the multiple secretomes secreted from transplanted DPSCs or directly injected DPSC-SFs in the early phase of transplantation/administration.
登录
查看更多内容
影响因子：
3.2
作者：
Omi M;Hata M;Nakamura N;Miyabe M;Kobayashi Y;Kamiya H;Nakamura J;Ozawa S;Tanaka Y;Takebe J;Matsubara T;Naruse K
通讯作者：
Naruse K
影响因子：
3.3
作者：
Okawa, Tetsuji;Kamiya, Hideki;Isobe, Ken-Ichi
通讯作者：
Isobe, Ken-Ichi
DOI：
10.1002/stem.2578
发表时间：
2017-05
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Garbuzova-Davis S;Haller E;Lin R;Borlongan CV
通讯作者：
Borlongan CV
影响因子：
4.3
作者：
Himeno T;Kamiya H;Naruse K;Cheng Z;Ito S;Shibata T;Kondo M;Kato J;Okawa T;Fujiya A;Suzuki H;Kito T;Hamada Y;Oiso Y;Isobe K;Nakamura J
通讯作者：
Nakamura J
影响因子：
16.2
作者：
Hotta, Nigishi;Akanuma, Yasuo;Shigeta, Yukio
通讯作者：
Shigeta, Yukio