Complete nucleotide sequence of reovirus L2 gene and deduced amino acid sequence of viral mRNA guanylyltransferase.
Complete nucleotide sequence of reovirus L2 gene and deduced amino acid sequence of viral mRNA guanylyltransferase.
复制标题
呼肠孤病毒L2基因的完整核苷酸序列和推导的病毒mRNA鸟苷酸转移酶的氨基酸序列。
DOI：
10.1016/s0021-9258(18)49252-6
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
AJ Shatkin
中科院分区：
文献类型：
--
作者：
LS Seliger;K. Zheng;AJ Shatkin
Reovirus mRNAs synthesized by the virion-associated RNA polymerase contain a 5′-terminal cap that is added to nascent transcripts by polypeptide lambda 2, a structural component of virions encoded by double-stranded RNA genome segment L2. The complete, 3916-nucleotide sequence of a full-length reovirus type 3 L2 DNA clone was determined by the dideoxy chain terminator method. The sequence has a single long open reading frame extending from the second A-T-G at nucleotide 14 to a termination codon at position 3881. On this basis, the 1289-amino acid sequence of polypeptide lambda 2, the reovirus mRNA guanylyltransferase, was deduced and compared to other GTP-binding proteins. Two different, lysine-containing lambda 2 peptide sequences closely resemble predicted amino acid stretches in vaccinia virus guanylyltransferase and potentially form part of active sites in the viral mRNA capping enzymes.
