Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz
Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz
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DOI：
10.3390/ijms20102596
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发表时间：
2019-05
影响因子：
5.6
通讯作者：
Quanliang Xie;G. Ding;Liping Zhu;Li Yu;Boxuan Yuan;Xuan Gao;Dan Wang;Yong Sun;Yang Liu-
中科院分区：
文献类型：
--
作者：
Quanliang Xie;G. Ding;Liping Zhu;Li Yu;Boxuan Yuan;Xuan Gao;Dan Wang;Yong Sun;Yang Liu-
橡胶草蒲公英（Taraxacum kok-saghyz，TKS）在其膨大的根部含有大量的天然橡胶（顺式-1，4-聚异戊二烯），是天然橡胶的替代作物来源。天然橡胶的生物合成和贮藏是一个涉及多个基因、蛋白质及其辅助因子的级联过程。TKS基因组刚刚被注释，许多NRB相关基因已被确定。然而，目前对TKS根中NRB的蛋白质调控机制的认识有限。采用双向凝胶电泳（2-DE）和质谱（MS）相结合的方法，从川牛膝成熟根中鉴定出371种蛋白质。同时，在扩大阶段的TKS根蛋白质进行了大规模的鸟枪分析，并确定了3545个蛋白质。随后，对2-DE凝胶电泳和鸟枪质谱鉴定的TKS根蛋白进行了基因本体和京都基因与基因组百科全书（KEGG）富集分析，发现大多数蛋白参与碳代谢过程，在膜结合细胞器中具有催化活性，其次是具有结合能力、运输和苯丙素类生物合成活性的蛋白。从TKS根中鉴定出58个NRB相关蛋白，包括8个小橡胶颗粒蛋白（SRPP）和2个橡胶延伸因子（REF）成员，这些蛋白参与甲羟戊酸（MVA）和甲羟戊酸磷酸（MEP）途径。据我们所知，这是第一个高分辨率草案蛋白质组图谱的成熟TKS根。TKS根的蛋白质组学研究表明MVA和MEP途径对NRB都很重要，SRPP可能比REF更重要。这些发现不仅加深了我们对TKS根蛋白质组的理解，而且为这些NRB相关蛋白在成熟TKS根中的作用提供了新的证据。
The rubber grass Taraxacum kok-saghyz (TKS) contains large amounts of natural rubber (cis-1,4-polyisoprene) in its enlarged roots and it is an alternative crop source of natural rubber. Natural rubber biosynthesis (NRB) and storage in the mature roots of TKS is a cascade process involving many genes, proteins and their cofactors. The TKS genome has just been annotated and many NRB-related genes have been determined. However, there is limited knowledge about the protein regulation mechanism for NRB in TKS roots. We identified 371 protein species from the mature roots of TKS by combining two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). Meanwhile, a large-scale shotgun analysis of proteins in TKS roots at the enlargement stage was performed, and 3545 individual proteins were determined. Subsequently, all identified proteins from 2-DE gel and shotgun MS in TKS roots were subject to gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and most proteins were involved in carbon metabolic process with catalytic activity in membrane-bounded organelles, followed by proteins with binding ability, transportation and phenylpropanoid biosynthesis activities. Fifty-eight NRB-related proteins, including eight small rubber particle protein (SRPP) and two rubber elongation factor(REF) members, were identified from the TKS roots, and these proteins were involved in both mevalonate acid (MVA) and methylerythritol phosphate (MEP) pathways. To our best knowledge, it is the first high-resolution draft proteome map of the mature TKS roots. Our proteomics of TKS roots revealed both MVA and MEP pathways are important for NRB, and SRPP might be more important than REF for NRB in TKS roots. These findings would not only deepen our understanding of the TKS root proteome, but also provide new evidence on the roles of these NRB-related proteins in the mature TKS roots.