INHIBITION OF VIRAL GENE-EXPRESSION BY THE CATALYTIC RNA SUBUNIT OF RNASE-P FROM ESCHERICHIA-COLI
INHIBITION OF VIRAL GENE-EXPRESSION BY THE CATALYTIC RNA SUBUNIT OF RNASE-P FROM ESCHERICHIA-COLI
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DOI：
10.1101/gad.9.4.471
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发表时间：
1995-02-15
影响因子：
10.5
通讯作者：
ALTMAN, S
中科院分区：
文献类型：
--
作者：
LIU, FY;ALTMAN, S
来自大肠杆菌的RNase P的催化RNA亚基（M1 RNA）已转化为内切核糖核酸酶，其特异性切割编码单纯疱疹病毒1（HSV-1）胸苷激酶（TK）的mRNA。与TK mRNA互补序列的M1 RNA的3'端共价连接导致靶RNA在体外非常有效的切割。该反应可被从E. coli和HeLa细胞。当用HSV-1感染培养物中表达新型RNA构建体的小鼠细胞时，与不表达新型RNA构建体或表达具有已知消除M1 RNA催化活性的某些缺失的构建体的细胞相比，TK mRNA和蛋白质的水平均降低约80%。
The catalytic RNA subunit (M1 RNA) of RNase P from Escherichia coli has been converted to an endoribonuclease that specifically cleaves the mRNA that encodes thymidine kinase (TK) of herpes simplex virus 1 (HSV-1). Covalent attachment to the 3' end of M1 RNA of a sequence complementary to TK mRNA results in very efficient cleavage of the target RNA in vitro. This reaction can be stimulated by proteins extracted from both E. coli and HeLa cells. When mouse cells in culture that express the novel RNA construct are infected with HSV-1, the levels of both TK mRNA and protein are reduced by similar to 80% as compared with cells that either do not express the novel RNA construct or express constructs with certain deletions that are known to abolish the catalytic activity of M1 RNA.