The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase
The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase
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DOI：
10.1073/pnas.94.26.14326
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发表时间：
1997-12-23
影响因子：
11.1
通讯作者：
Rosen, BP
中科院分区：
文献类型：
--
作者：
Rensing, C;Mitra, B;Rosen, BP
第一个Zn（II）易位的P型ATP酶已被鉴定为o 732的产物，o 732是在大肠杆菌基因组测序中鉴定的潜在基因，该基因称为zntA，通过同源重组插入卡那霉素基因而被破坏。该突变株对锌和镉盐表现出超敏反应，但对其他金属盐不敏感，表明其在E.杆菌从野生型菌株外翻膜囊泡积累Zn-65（II）和Cd-109（II），通过使用ATP作为能源，运输是敏感的钒酸盐，P型ATP酶的抑制剂。来自zntA：：kan菌株的膜囊泡不积累这些金属离子，突变体的敏感表型和转运缺陷都通过在质粒上表达zntA来补充。
The first Zn(II)-translocating P-type ATPase has been identified as the product of o732, a potential gene identified in the sequencing of the Escherichia coli genome, This gene, termed zntA, was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain exhibited hypersensitivity to zinc and cadmium salts but not salts of other metals, suggesting a role in zinc homeostasis in E. coli. Everted membrane vesicles from a wild-type strain accumulated Zn-65(II) and Cd-109(II) by using ATP as an energy source, Transport was sensitive to vanadate, an inhibitor of P-type ATPases. Membrane vesicles from the zntA::kan strain did not accumulate those metal ions, Both the sensitive phenotype and transport defect of the mutant were complemented by expression of zntA on a plasmid.