Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms
Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms
复制标题
通过转基因蚕内源抗病毒基因的过度表达来抵抗家蚕核多角体病毒
DOI：
10.1007/s00705-012-1309-8
复制
发表时间：
2012-07-01
影响因子：
2.7
通讯作者：
Xia, Qingyou
中科院分区：
文献类型：
--
作者：
Jiang, Liang;Wang, Genhong;Xia, Qingyou
转基因技术是改良抗病物种的有力工具。从家蚕中肠汁中分离纯化的Bmlipase-1具有较强的抗病毒活性。家蚕核型多角体病毒(BmNPV)为了培育抗病毒家蚕品种，在杆状病毒IE1启动子的控制下，构建了高表达Bmlipase-1基因的转基因载体。通过胚胎显微注射获得转基因株系。转基因品系中肠Bmlipase-1的mRNA水平比非转基因品系高27.3%。用不同剂量的BmNPV饲喂家蚕后，当病毒剂量为1060B/头时，转基因家蚕的死亡率比非转基因家蚕降低约33.6%。这些结果表明，过表达内源抗病毒基因可以增强家蚕对病毒的抗性。
Transgenic technology is a powerful tool for improving disease-resistant species. Bmlipase-1, purified from the midgut juice ofBombyx mori, showed strong antiviral activity againstB. morinucleopolyhedrovirus (BmNPV). In an attempt to create an antiviral silkworm strain for sericulture, a transgenic vector overexpressing theBmlipase-1gene was constructed under the control of a baculoviral immediate early-1 (IE1) promoter. Transgenic lines were generated via embryo microinjection. The mRNA level ofBmlipase-1in the midguts of the transgenic line was 27.3 % higher than that of the non-transgenic line. After feeding the silkworm with different amounts of BmNPV, the mortality of the transgenic line decreased to approximately 33 % compared with the non-transgenic line when the virus dose was 106OB/larva. These results imply that overexpressing endogenous antiviral genes can enhance the antiviral resistance of silkworms.