Transcriptional and Translational Differences of Microglia from Male and Female Brains
Transcriptional and Translational Differences of Microglia from Male and Female Brains
复制标题
DOI：
10.1016/j.celrep.2018.08.001
复制
发表时间：
2018-09-04
期刊：
影响因子：
8.8
通讯作者：
Wolf, Susanne A.
中科院分区：
文献类型：
--
作者：
Guneykaya, Dilansu;Ivanov, Andranik;Wolf, Susanne A.
大脑结构和功能的性别差异具有重要的科学意义，因为与性别相关的精神和神经疾病易感性。神经炎症是许多这些疾病的共同特征，因此小胶质细胞作为大脑的免疫能力细胞，已经成为性别特异性研究的焦点。在这里，我们展示了从雄性和雌性小鼠大脑中新分离的小胶质细胞的结构、功能、转录组学和蛋白质组学特征的差异。我们发现，男性小胶质细胞在特定的大脑区域更常见，具有更高的抗原呈递能力，并且似乎对ATP等刺激有更高的反应潜力，这反映在更高的基线外向和内向电流以及更高的嘌呤能受体蛋白表达上。总之，我们提供了一个全面的资源来产生和验证关于大脑性别差异的假设。
Sex differences in brain structure and function are of substantial scientific interest because of sex-related susceptibility to psychiatric and neurological disorders. Neuroinflammation is a common denominator of many of these diseases, and thus microglia, as the brain's immunocompetent cells, have come into focus in sex-specific studies. Here, we show differences in the structure, function, and transcriptomic and proteomic profiles in microglia freshly isolated from male and female mouse brains. We show that male microglia are more frequent in specific brain areas, have a higher antigen-presenting capacity, and appear to have a higher potential to respond to stimuli such as ATP, reflected in higher baseline outward and inward currents and higher protein expression of purinergic receptors. Altogether, we provide a comprehensive resource to generate and validate hypotheses regarding brain sex differences.