Cyclic AMP stabilizes a class of developmentally regulated Dictyostelium discoideum mRNAs
Cyclic AMP stabilizes a class of developmentally regulated Dictyostelium discoideum mRNAs
复制标题
环 AMP 可稳定一类发育调节的盘基网柄菌 mRNA
作者：
G. Mangiarotti;A. Ceccarelli;H. Lodish
mRNA的稳定性是调节蛋白质合成的重要方面。在哺乳动物细胞中，大多数mRNA具有长半衰期（5-15小时），但相当大一部分不太稳定1，2。很少有特定mRNA或一类mRNA的稳定性受到环境或发育刺激的影响。某些激素引起mRNA种类的特异性稳定化3，并且优先mRNA稳定性分别在红细胞生成或肌生成的终末阶段珠蛋白和肌球蛋白mRNA的积累中是重要的4 -7。盘基网柄藻聚集体的解聚诱导了一大类发育调控mRNA的特异性去稳定化;因此，该系统是确定此类控制如何起作用的极好系统8 -10。在这里，我们表明，除了环AMP解聚细胞特异性地防止这些mRNA的不稳定。
The stability of mRNA is an important facet of the regulation of protein synthesis. In mammalian cells most mRNAs have long half-lives (5–15 hours) but a substantial fraction are much less stable1,2. There are few examples where the stability of a particular mRNA or class of mRNAs is specifically affected by environmental or developmental stimuli. Certain hormones cause specific stabilization of mRNAs species3 and preferential mRNA stability is important in the accumulation of globin and myosin mRNAs during the terminal stages of erythropoesis or myogenesis, respectively4–7. Disaggregation of Dictyostelium discoideum aggregates induces the specific destabilization of a large class of developmentally regulated mRNAs; thus, this system is an excellent one in which to determine how such controls are effected8–10. Here we show that addition of cyclic AMP to disaggregated cells specifically prevents the destabilization of these mRNAs.