2.8-A crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop
2.8-A crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop
复制标题
大肠杆菌 YidC 的 2.8-A 晶体结构揭示了所有核心区域，包括柔性 C2 环
DOI：
10.1016/j.bbrc.2018.09.043
复制
发表时间：
2018
影响因子：
3.1
通讯作者：
Tsukazaki Tomoya
中科院分区：
文献类型：
--
作者：
Tanaka Yoshiki;Izumioka Akiya;Abdul Hamid Aisyah;Fujii Akira;Haruyama Takamitsu;Furukawa Arata;Tsukazaki Tomoya
YidC/Alb 3/Oxa 1家族蛋白参与膜蛋白的插入和组装。YidC的核心五个跨膜区在蛋白质家族中是保守的，形成了向细胞质侧开放的带正电荷的空腔。空腔在膜蛋白插入中起重要作用。在所有报道的YidC结构研究中，第二个胞质环（C2环）是无序的，限制了对其作用的理解。在这里，我们确定了YidC的晶体结构，包括C2环，分辨率为2.8 μ m，R/Rfree= 21.8/27.5。这种结构和随后的分子动力学模拟表明，内在的柔性C2环覆盖带正电的腔。该晶体结构提供了包括C2环的完整核心区域的坐标，这对于YidC的进一步分析是有价值的。
YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open to the cytoplasmic side. The cavity plays an important role in membrane protein insertion. In all reported structural studies of YidC, the second cytoplasmic loop (C2 loop) was disordered, limiting the understanding of its role. Here, we determined the crystal structure of YidC including the C2 loop at 2.8 Å resolution withR/Rfree= 21.8/27.5. This structure and subsequent molecular dynamics simulation indicated that the intrinsic flexible C2 loop covered the positively charged cavity. This crystal structure provides the coordinates of the complete core region including the C2 loop, which is valuable for further analyses of YidC.