Inhibiting effects of resveratrol and its glucoside piceid against Venturia inaequalis, the causal agent of apple scab
Inhibiting effects of resveratrol and its glucoside piceid against Venturia inaequalis, the causal agent of apple scab
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DOI：
10.1021/jf048375h
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发表时间：
2005-01-26
影响因子：
6.1
通讯作者：
Szankowski, I
中科院分区：
文献类型：
--
作者：
Schulze, K;Schreiber, L;Szankowski, I
白藜芦醇是一种植物抗毒素，产生于不同的无关植物物种，如葡萄藤和花生。以苹果（Malus arteritica Borkh.）表皮细胞膜为材料，研究了白藜芦醇及其糖苷反式白藜芦醇-3-O-β-D-吡喃葡萄糖苷（白藜芦醇苷）对苹果黑星病菌（Venturia inaequalis）的防治效果。叶子测试物质白藜芦醇和白藜芦醇苷在接种孢子之前被吸附到CM上，或者与黑星病菌孢子同时被施加到CM上，并检查它们对发芽、附着胞形成和渗透的影响。白藜芦醇对孢子萌发没有影响，但同时施用和施用前都有显著的抑制作用。同时给药和给药前分别观察到89.7 +/- 11.5和61.8 +/- 135.1的抑制百分比。白藜芦醇葡萄糖苷白藜芦醇苷对发芽有显著的抑制作用，并且当与孢子同时施加到CM时，在200和400 μ g mL（-1）之间的浓度下完全抑制真菌的渗透。在富含云杉色素的CM上（预施用），孢子萌发，但渗透几乎完全被抑制（96.1 +/- 5.1%）。因此，离体实验表明，白藜芦醇及其糖苷确实有助于提高苹果叶片的抗病性。
Resveratrol is a phytoalexin produced in different unrelated plant species such as grapevine and peanut. The effect of resveratrol and its glucoside trans-resveratrol-3-O-beta-D-glycopyranosid (piceid) against Venturia inaequalis, the causal agent of apple scab, was evaluated using a newly established test based on enzymatically isolated cuticular membranes (CMs) from apple (Malus domestica Borkh.) leaves. The test substances resveratrol and piceid were either sorbed to CMs before inoculation with spores or were applied simultaneously with the Venturia inaequalis spores to the CMs, and their effect on germination, appressoria formation, and penetration was examined. Resveratrol had no influence on spore germination but a significant inhibiting effect on penetration when applied simultaneously as well as before. A percentage inhibition of 89.7 +/- 11.5 and 61.8 +/- 135.1 was observed for simultaneous and preapplication, respectively. The resveratrol glucoside piceid had a significant inhibitory effect on germination and completely inhibited penetration of the fungus at concentrations between 200 and 400 mug mL(-1) when applied simultaneously with the spores to the CMs. On piceid-enriched CM (preapplication), spores germinated but penetration was inhibited nearly completely (96.1 +/- 5.1%). Thus, in vitro experiments showed that resveratrol and its glucoside in fact could contribute to improving the pathogen resistance of apple leaves.