Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate
Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate
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DOI：
10.1128/aem.62.10.3800-3808.1996
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发表时间：
1996-10
影响因子：
4.4
通讯作者：
Robert A. Sanford;James R. Cole;Frank E. Löffler;J. Tiedje
中科院分区：
文献类型：
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作者：
Robert A. Sanford;James R. Cole;Frank E. Löffler;J. Tiedje
菌株Co23是一种厌氧产芽孢微生物，因其能够以2,3-二氯苯酚（DCP）作为电子受体生长，从多取代酚中去除邻氯，但不能从单卤代酚中去除邻氯，从而从堆肥土壤中富集并分离出菌株Co23。在 3-氯-4-羟基苯甲酸酯存在下，从乳酸盐氧化成乙酸盐，每摩尔还原当量（如 2[H]）的生长产量为 3.24 g 细胞，表明通过氯呼吸进行生长，但在不存在卤代电子受体的情况下不生长。氯呼吸的其他指标是用于脱氯的电子供体的电子分数 (0.67) 和 < 1.0 ppm 的 H2 阈值浓度。用于还原脱卤的其他电子供体是丙酮酸、甲酸、丁酸、巴豆酸和H 2 。在没有电子受体的情况下，丙酮酸支持同型产乙酸生长。菌株Co23也使用亚硫酸盐、硫代硫酸盐和硫作为生长的电子受体，但它不使用硫酸盐、硝酸盐或富马酸盐。最适生长温度为37℃；然而，脱氯率在45℃时达到最佳，并且活性持续到高达55℃。测定16S rRNA序列，发现菌株Co23与脱硫杆菌脱卤JW/IU DC1和脱硫杆菌菌株PCE1相关，序列相似性分别为97.2%和96.8%。菌株 Co23 表现出的系统发育和生理学特性将其归入一个新物种，命名为脱硫杆菌（Desulfitobacter chromorespirans）。
Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor, ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlororespiration was indicated by a growth yield of 3.24 g of cells per mol of reducing equivalents (as 2[H]) from lactate oxidation to acetate in the presence of 3-chloro-4-hydroxybenzoate but no growth in the absence of the halogenated electron acceptor. Other indicators of chlororespiration were the fraction of electrons from the electron donor used for dechlorination (0.67) and the H2 threshold concentration of < 1.0 ppm. Additional electron donors utilized for reductive dehalogenation were pyruvate, formate, butyrate, crotonate, and H2. Pyruvate supported homoacetogenic growth in the absence of an electron acceptor. Strain Co23 also used sulfite, thiosulfate, and sulfur as electron acceptors for growth, but it did not use sulfate, nitrate or fumarate. The temperature optimum for growth was 37 degrees C; however, the rates of dechlorination were optimum at 45 degrees C and activity persisted to temperatures as high as 55 degrees C. The 16S rRNA sequence was determined, and strain Co23 was found to be related to Desulfitobacterium dehalogenans JW/IU DC1 and Desulfitobacterium strain PCE1, with sequence similarities of 97.2 and 96.8%, respectively. The phylogenetic and physiological properties exhibited by strain Co23 place it into a new species designated Desulfitobacterium chlororespirans.