Cellular Imaging Using Stimulated Raman Scattering Microscopy
Cellular Imaging Using Stimulated Raman Scattering Microscopy
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DOI：
10.1021/acs.analchem.9b02095
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发表时间：
2019-08-06
影响因子：
7.4
通讯作者：
Fu, Dan
中科院分区：
文献类型：
--
作者：
Hill, Andrew H.;Fu, Dan
细胞成像是一个活跃的研究领域，使研究人员能够监测细胞动力学，以及对各种外部刺激（生理应激，外源性化合物等）的反应。受激拉曼散射（SRS）显微镜是一种流行的实验工具，用于成像细胞，主要是因为它的化学特异性，高空间分辨率，和高图像采集速度。在这个角度来看，SRS显微镜的理论背景和实验实现进行了讨论，并在SRS细胞成像领域的最新进展突出和总结。
Cellular imaging is an active area of research that enables researchers to monitor cellular dynamics, as well as responses to various external stimuli (physiological stress, exogenous compounds, etc.). Stimulated Raman scattering (SRS) microscopy is one popular experimental tool used to image cells, largely because of its chemical specificity, high spatial resolution, and high image acquisition speed. In this Perspective, the theoretical background and experimental implementation of SRS microscopy are discussed and recent developments in the field of cellular imaging with SRS are highlighted and summarized.