Luteotrophic properties of some pituitary hormones in nonpregnant or pregnant hypophysectomized ewes.
Luteotrophic properties of some pituitary hormones in nonpregnant or pregnant hypophysectomized ewes.
复制标题
未怀孕或怀孕垂体切除母羊中某些垂体激素的促黄体营养特性。
作者：
C. Kaltenbach;J. Graber;G. Niswender;A. Nalbandov
周期性或妊娠绵羊垂体切除导致黄体（CL）立即和完全变性，因此表明绵羊CL需要垂体促黄体物质的形成和维持。以前的尝试，以维持CL垂体切除母羊与完整的子宫失败时，注射各种激素。本研究提供的数据表明，含有FSH和LH以及其他垂体激素的垂体粗提物在垂体切除的非妊娠或妊娠绵羊中具有促黄体作用。单独的粗LH也是促黄体的，而纯化的LH只是部分促黄体的，因为它不允许CL完全变性，并且只能维持妊娠有限的时间。相反，FSH，催乳素，或雌激素单独显示垂体切除动物没有促黄体功能。在这些实验中，粗LH维持CL的原因在于它被持续输注。据估计，LH/hr实际值远低于1 μg。
Hypophysectomy of cyclic or pregnant sheep causes immediate and complete degeneration of the corpus luteum (CL), thus indicating that sheep CL need a hypophysial luteotrophic substance for formation and maintenance. Previous attempts to maintain CL in hypophysectomized ewes with intact uteri have failed when various hormones were injected. The present study presents data which show that crude pituitary gland extracts containing both FSH and LH, as well as other hypophysial hormones, are luteotrophic in hypophysectomized nonpregnant or pregnant sheep. Crude LH alone is also luteotrophic, while purified LH is only partially luteotrophic in that it does not allow complete degeneration of CL, and can maintain pregnancy for only a limited time. In contrast, FSH, prolactin, or estrogen alone show no luteotrophic properties in hypophysectomized animals. The reason crude LH maintained CL in these experiments lies in the fact that it was infused constantly. It is estimated that much less than 1 μg of LH/hr actuall...