Mu heavy chains can associate with a pseudo-light chain complex (psi L) in human pre-B cell lines.
Mu heavy chains can associate with a pseudo-light chain complex (psi L) in human pre-B cell lines.
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DOI：
10.1093/intimm/1.4.355
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发表时间：
1989-01-01
影响因子：
4.4
通讯作者：
Hendershot, L M
中科院分区：
文献类型：
--
作者：
Kerr, W G;Cooper, M D;Hendershot, L M
在前 B 细胞中，即 B 细胞分化的最早可识别阶段，存在免疫球蛋白链表达的异步，因为 mu 重链是在没有轻链合成的情况下合成的。这些 mu 链大部分保留在细胞内并被降解。在这里，我们证明人类前 B 细胞系中的一部分 mu 链可以与相对分子质量分别为 22、18 和 16 kd 的三种前 B 特异性蛋白结合到达表面，我们将其统称为伪轻链复合物 psi L。这种关联产生多聚体复合物 mu 2-psi L。其中两个 psi L 蛋白（22 和 16 kd）具有 lambda 免疫反应性，并与 mu 链形成二硫键，这表明它们与传统的 lambda 轻链密切相关。 18 kd psi L 物种是复合物的非共价缔合成员。 mu-psi L 复合物在前 B 细胞表面的表达可能在造血微环境控制前 B 生长和分化中发挥功能作用。
In pre-B cells, the earliest identifiable stage of B cell differentiation, there is an asynchrony of immunoglobulin chain expression in that mu heavy chains are synthesized in the absence of light chain synthesis. These mu chains largely remain intracellular and are degraded. Here we demonstrate that a fraction of mu chains in human pre-B cell lines can reach the surface in association with three pre-B-specific proteins with relative molecular masses of 22, 18, and 16 kd, which we term collectively the pseudo-light chain complex, psi L. This association generates a multimeric complex, mu 2-psi L. Two of the psi L proteins (22 and 16 kd) are lambda-immunoreactive and form disulfide bonds with mu chains, suggesting that they are closely related to conventional lambda light chains. The 18 kd psi L species is a non-covalently-associated member of the complex. The expression of mu-psi L complexes on the surface of pre-B cells could have a functional role in the control of pre-B growth and differentiation by the hematopoietic microenvironment.