Raman microspectroscopy for microbiology
Raman microspectroscopy for microbiology
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DOI：
10.1038/s43586-021-00075-6
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发表时间：
2021-11-25
期刊：
影响因子：
--
通讯作者：
Stocker, Roman
中科院分区：
文献类型：
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作者：
Lee, Kang Soo;Landry, Zachary;Stocker, Roman
拉曼显微光谱学为微生物学家提供了一种快速、非破坏性的技术，可以近乎实时地评估单个活微生物的化学成分。在本入门中，我们概述了其方法及其在微生物学中的应用潜力。我们描述了拉曼分析的技术方面和实际的方法，将这种方法应用于微生物问题。我们讨论了最近和潜在的未来应用，以确定微生物代谢物的组成和分布到亚细胞尺度；研究宿主-微生物、细胞-细胞和细胞-环境的分子交换，这些分子交换构成了从海洋到人类肠道微生物群落的微生物生态系统结构；并询问在环境和工业过程中功能角色的微生物多样性-现代微生物学的关键主题。我们描述了目前的技术限制，拉曼显微光谱研究微生物和方法，以尽量减少或解决他们。拉曼显微光谱学的最新技术创新将进一步加强该方法在微生物学中更广泛应用的力量和能力，使微生物学家能够在几乎任何感兴趣的尺度上加深对复杂群落微生物生态学的理解。
Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to assess the chemical composition of individual live microorganisms in near real time. In this Primer, we outline the methodology and potential for its application to microbiology. We describe the technical aspects of Raman analyses and practical approaches to apply this method to microbiological questions. We discuss recent and potential future applications to determine the composition and distribution of microbial metabolites down to subcellular scale; to investigate the host-microorganism, cell-cell and cell-environment molecular exchanges that underlie the structure of microbial ecosystems from the ocean to the human gut microbiomes; and to interrogate the microbial diversity of functional roles in environmental and industrial processes - key themes in modern microbiology. We describe the current technical limitations of Raman microspectroscopy for investigation of microorganisms and approaches to minimize or address them. Recent technological innovations in Raman microspectroscopy will further reinforce the power and capacity of this method for broader adoptions in microbiology, allowing microbiologists to deepen their understanding of the microbial ecology of complex communities at nearly any scale of interest.