Quality control of laboratory methods for semen evaluation in a multicenter research study.
Quality control of laboratory methods for semen evaluation in a multicenter research study.
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多中心研究中精液评估实验室方法的质量控制。
DOI：
10.1002/j.1939-4640.2004.tb02836.x
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发表时间：
2004
影响因子：
--
通讯作者：
StudyforFutureFamiliesResearchGroup
中科院分区：
文献类型：
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作者：
Brazil,Charlene;Swan,ShannaH;Tollner,CharleneR;Treece,Cathy;Drobnis,ErmaZ;Wang,Christina;Redmon,JBruce;Overstreet,JamesW;StudyforFutureFamiliesResearchGroup
严格标准化的实验室协议和严格的质量控制（QC）对于多个地点精液质量数据之间的有意义的比较至关重要。我们描述了我们的经验与未来的家庭研究（SFF），在美国的精液质量的多中心研究。制定了详细的方案，每个研究中心的技术人员参加了中心实验室的培训课程。技术人员从稀释的精液标本中获得盲法重复样本，用于MicroCell和血细胞计数器计数。根据世界卫生组织（WHO）的建议，使用录像记录的简单活力百分比和个体精子进展的分类评估来评估精子活力。单个标本的平均技术员间变异系数为12.6%（微细胞计数）、15.2%（血细胞计数）和10.5%（活力百分比）。MicroCell计数的技术员内变异系数平均为10.3%，血细胞计数为12.5%，运动百分比为5.2%。对于MicroCell计数、血细胞计数和简单活力百分比，技术人员的值与单个标本的中心标准值之间的平均百分比差异分别为13.5%、16.6%和11.9%。我们实现了我们的目标，保持平均技术人员内变异系数和平均百分比差异的标准值为15%或更少的简单百分比的运动力和精子浓度的测量微细胞。使用改进的Neubauer血细胞计数器室进行标准化证明更加困难。我们没有成功地标准化一种方法来分类评估个体精子的进展。
Rigorously standardized laboratory protocols and strict quality control (QC) are essential for meaningful comparisons between semen quality data from multiple sites. We describe our experience with the Study for Future Families (SFF), a multicenter study of semen quality in the United States. Detailed protocols were developed, and technicians from each study site attended a training session at the central laboratory. Technicians received blinded replicates from diluted semen specimens for counting by MicroCell and hemacytometer. Sperm motility was assessed using videotaped recordings for simple percent motility and categorical assessment of individual sperm progression as recommended by the World Health Organization (WHO). The mean intertechnician coefficient of variation for individual specimens was 12.6% for MicroCell counts, 15.2% for hemacytometer counts, and 10.5% for percent motility. Intratechnician coefficients of variation averaged 10.3% for MicroCell counts, 12.5% for hemacytometer counts, and 5.2% for percent motility. The average percent differences between the technicians' values and the central standard for individual specimens were 13.5%, 16.6%, and 11.9% for MicroCell counts, hemacytometer counts, and simple percent motility, respectively. We achieved our goal of maintaining mean intratechnician coefficients of variation and mean percent differences from the standard values of 15% or less for measurements of simple percent motility and sperm concentration by MicroCell. Standardization using the Improved Neubauer hemacytometer chamber proved more difficult. We were not successful in standardizing a method for categorical assessment of individual sperm progression.