Artifacts Related to Sample Introduction in Capillary Gel Electrophoresis Affecting Separation Performance and Quantitation
Artifacts Related to Sample Introduction in Capillary Gel Electrophoresis Affecting Separation Performance and Quantitation
复制标题
与影响分离性能和定量的毛细管凝胶电泳进样相关的人为因素
DOI：
10.1021/ac00109a056
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发表时间：
1995
影响因子：
7.4
通讯作者：
H. Schwartz
中科院分区：
文献类型：
--
作者：
A. Guttman;H. Schwartz
报告了毛细管凝胶电泳 (CGE) 的两个与注射相关的伪影。第一种情况发生在连续进样相同的小体积 (10-200 µL) 水性样品时：每次进样都会获得逐渐变小的峰高。通过检查动电样品引入过程中发生的电化学过程，可以定性地解释这种现象。该问题的一个简单解决方案是在样品注入之前从水瓶中进行中间电动注入。这种两步进样程序不仅可以显着提高精度（对于定量研究很重要），而且可以增加样品加载量。在 CGE 中观察到的第二个伪影与凝胶填充毛细管入口的物理形状有关：使用斜边毛细管会导致性能差、峰失真和分辨率损失。
Two injection-related artifacts with capillary gel electrophoresis (CGE) are reported. The first occurs with consecutive injections from the same, low-volume (10-200 μL), aqueous sample : progressively smaller peak heights are obtained with each injection. This phenomenon is explained in qualitative terms by examining the electrochemical processes occurring during the electrokinetic sample introduction. A simple solution to this problem is to perform an intermediate electrokinetic injection from a water vial prior to the sample injection. This two-step injection procedure results not only in dramatically increased precision (important for quantitative studies) but also in increased sample loading. The second artifact observed in CGE is related to the physical shape of the inlet of the gel-filled capillary : employing an oblique edge capillary results in poor performance, peak distortion, and loss in resolution.
影响因子：
7.4
作者：
FISHMAN, HA;AMUDI, NM;ZARE, RN
通讯作者：
ZARE, RN