Live-pig-airway surface imaging and whole-pig CT at the Australian Synchrotron Imaging and Medical Beamline
Live-pig-airway surface imaging and whole-pig CT at the Australian Synchrotron Imaging and Medical Beamline
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DOI：
10.1107/s1600577518014133
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发表时间：
2019-01-01
影响因子：
2.5
通讯作者：
Parsons, David W.
中科院分区：
文献类型：
--
作者：
Donnelley, Martin;Morgan, Kaye S.;Parsons, David W.
澳大利亚同步加速器成像和医疗光束线（IMBL）被设计成世界上最宽的同步加速器X射线束，部分用于人类的临床成像和治疗应用，以及大型动物模型的成像。我们的团队目前对新开发的囊性纤维化（CF）动物模型的气道成像感兴趣，这些动物模型显示类似人类的肺部疾病，例如CF猪。评估CF气道治疗有效性的一个关键结局指标是肺通过粘膜纤毛转运（MCT）清除吸入颗粒的能力。这项研究扩展了作者先前在IMBL进行的羊和猪气管组织离体研究。在本研究中，试图确定IMBL的设计是否适合于生猪气管MCT成像。对沉积在8只活仔猪气管气道表面上的200 μ m直径高折射率（HRI）玻璃珠标记物颗粒的移动进行了跟踪和定量，并检查了MCT对气雾剂给药的反应。还对一头猪进行了高分辨率计算机断层扫描（CT）全动物尸检扫描，以验证IMBL的大样本CT能力。MCT跟踪颗粒在所有动物中均可见，所用的自动MCT跟踪算法能够识别和跟踪许多颗粒，但当颗粒移动速度超过约6 mmmin（-1）（暴露之间50像素）或颗粒接触或重叠时，准确度降低。根据CT数据集成功绘制了渲染图。由于技术问题，无法使用可靠的遮板，因此辐射剂量是可变的。由于剂量必须仔细控制在未来的研究中，估计使用本实验设计的最低可实现的辐射剂量。总之，本研究证明了IMBL适用于大动物气管MCT成像和整体动物CT。
The Australian Synchrotron Imaging and Medical Beamline (IMBL) was designed to be the world's widest synchrotron X-ray beam, partly to enable clinical imaging and therapeutic applications for humans, as well as for imaging large-animal models. Our group is currently interested in imaging the airways of newly developed cystic fibrosis (CF) animal models that display human-like lung disease, such as the CF pig. One key outcome measure for assessing the effectiveness of CF airway therapies is the ability of the lung to clear inhaled particulates by mucociliary transit (MCT). This study extends the ex vivo sheep and pig tracheal-tissue studies previously performed by the authors at the IMBL. In the present study, attempts were made to determine whether the design of the IMBL is suitable for imaging tracheal MCT in live pigs. The movement of 200 mu m-diameter high-refractive-index (HRI) glass-bead marker particles deposited onto the tracheal airway surface of eight live piglets was tracked and quantified and the MCT response to aerosol delivery was examined. A high-resolution computed tomographic (CT) whole-animal post-mortem scan of one pig was also performed to verify the large sample CT capabilities of the IMBL. MCT tracking particles were visible in all animals, and the automated MCT tracking algorithms used were able to identify and track many particles, but accuracy was reduced when particles moved faster than approximate to 6mmmin(-1) (50pixels between exposures), or when the particles touched or overlapped. Renderings were successfully made from the CT data set. Technical issues prevented use of reliable shuttering and hence radiation doses were variable. Since dose must be carefully controlled in future studies, estimates of the minimum achievable radiation doses using this experiment design are shown. In summary, this study demonstrated the suitability of the IMBL for large-animal tracheal MCT imaging, and for whole-animal CT.