Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries.
Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries.
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DOI：
10.2147/ijn.s155245
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发表时间：
2018
影响因子：
8
通讯作者：
Duque C
中科院分区：
文献类型：
--
作者：
Aida KL;Kreling PF;Caiaffa KS;Calixto GMF;Chorilli M;Spolidorio DM;Santos-Filho NA;Cilli EM;Duque C
防龋剂必须干扰变形链球菌在牙科生物膜中的黏附和增殖，而不会引起宿主毒性和细菌耐药性。天然物质，包括阳离子抗菌肽(CAMP)及其片段，如β-防御素-3肽片段(d1-23)，已被广泛研究。然而，cAMP的化学和物理稳定性可能会受到外界因素的影响，如温度和pH，从而缩短了抗菌活性的时间。为了克服上述缺点，本研究开发并表征了一种药物释放系统，并评价了其细胞毒性和抗变形链球菌生物膜的效果。LCS由油酸、polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl醇、Carbopol®974P和Carbopol®971P组成。用偏光显微镜(PLM)、流变学(粘弹性和流动特性)和体外生物粘附性分析了LCS。观察培养上皮细胞的存活情况。为进一步评价药物作用4和24 h后对变形链球菌生物被膜的作用效果，测定了药物作用后d1~23的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。PLM、流变学和体外生物粘附性试验表明，人工唾液稀释后，LCS的粘度和生物粘附性均有所增加。与处理4h相比，负载D_1-23的LCS系统在24 h后对变形链球菌生物被膜具有更好的抗菌活性，表现出累积效应。LCS和负载D1-23的LCS对人上皮细胞均无毒性。D123负载LCS是一种很有前途的防龋剂给药系统。
Anticaries agents must interfere with the adhesion of Streptococcus mutans and its proliferation in dental biofilm, without causing host toxicity and bacterial resistance. Natural substances, including cationic antimicrobial peptides (CAMPs) and their fragments, such as β-defensin-3 peptide fragment (D1–23), have been widely studied. However, the chemical and physical stability of CAMPs may be compromised by external factors, such as temperature and pH, reducing the period of antimicrobial activity. To overcome the aforementioned disadvantage, this study developed and character-ized a drug delivery system and evaluated the cytotoxicity and effect against S. mutans biofilm of a D1–23-loaded bioadhesive liquid crystalline system (LCS). LCS was composed of oleic acid, polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, Carbopol® 974P and Carbopol® 971P. LCS was analyzed by polarized light microscopy (PLM), rheology (viscoelasticity and flow properties) and in vitro bioadhesion. The viability of epithelial cells was evaluated. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against S. mutans were determined for D1–23 for further evaluation of the effect against S. mutans biofilm after 4 and 24 h of exposure to treatments. PLM, rheology, and in vitro bioadhesion tests showed that both viscosity and bioadhesion of LCS increased after it was diluted with artificial saliva. D1–23-loaded LCS system presented better activity against S. mutans biofilm after 24 h when compared to 4 h of treatment, showing a cumulative effect. Neither LCS nor D1–23-loaded LCS presented toxicity on human epithelial cells. D1–23-loaded LCS is a promising drug delivery system for the prevention of dental caries.