Size dependent disruption of tethered lipid bilayers by functionalized polystyrene nanoparticles.
Size dependent disruption of tethered lipid bilayers by functionalized polystyrene nanoparticles.
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DOI：
10.1016/j.bbamem.2014.09.014
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Yuanfang Liu;R. Mark Worden
中科院分区：
文献类型：
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作者：
Yuanfang Liu;R. Mark Worden
工程纳米材料（ENM）和生物膜之间的分子相互作用还没有得到很好的理解。研究了粒径和表面官能团对聚苯乙烯纳米粒子（PNPs）生物膜破坏效能的影响。电化学阻抗谱用于测量PNP暴露后由1，2-二油酰-sn-甘油-磷酸胆碱（DOPC）组成的系留双层脂质膜BLM（tBLM）的电阻（Rm）的变化。所有测试的PNPs都引发了RM的下降，可以使用指数衰减模型进行描述。两个模型参数（指数速率常数和Rm的分数损失）的统计层次聚类分析可以区分基于大小和表面官能团的PNPs。对于COOH修饰的纳米颗粒，20 nm PNP比100 nm PNP更有效地降低Rm。然而，对于脒修饰的纳米颗粒，120 nm PNP在降低Rm方面比23 nm PNP更有效。COOH修饰的PNP在降低Rm方面比脒修饰的PNP更有效，后者在暴露于tBLM后倾向于聚集。超高效液相色谱-质谱分析表明，聚集可能是由脒PNP从tBLM中除去的DOPC引发的。
Molecular interactions between engineered nanomaterials (ENM) and biomembranes are not well understood. This study investigated the effects of particle size and surface functional group on polystyrene nanoparticles' (PNPs) potency for biomembrane disruption. Electrochemical impedance spectroscopy was used to measure changes in the electrical resistance (Rm) of a tethered bilayer lipid membrane BLM (tBLM) composed of 1,2-dioleoyl-sn-glycero-phosphocholine (DOPC) following PNP exposure. All PNPs tested triggered a decline in the Rmthat could be described using an exponential-decay model. Statistical hierarchical clustering analysis of two model parameters (exponential rate constant and fractional loss of Rm) could distinguish between the PNPs based on both size and surface functional group. For COOH modified nanoparticles, 20 nm PNPs were more potent in reducing Rmthan 100 nm PNP. However, for amidine modified nanoparticles, 120 nm PNPs were more potent in reducing Rmthan 23 nm PNP. The COOH modified PNPs were more potent in reducing Rmthan amidine modified PNP, which tended to aggregate following exposure to a tBLM. Ultra performance liquid chromatography–mass spectroscopy analysis suggested that the aggregation may have been triggered by DOPC that was removed from the tBLM by the amidine PNP.