Studying the influence of 3.1 THz irradiation on the endocytosis of neuronal cells
Studying the influence of 3.1 THz irradiation on the endocytosis of neuronal cells
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DOI：
10.1364/josab.439502
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发表时间：
2022-01-01
影响因子：
1.9
通讯作者：
Lu, Xiaoyun
中科院分区：
文献类型：
--
作者：
Hu, Erling;Wang, Leilei;Lu, Xiaoyun
随着太赫兹技术的迅速发展，人们越来越重视太赫兹电磁辐射的生物效应，但对太赫兹辐射对许多生物过程的影响尚不清楚。在本研究中，我们探讨了3.1太赫兹频率太赫兹辐射对神经元细胞内吞作用的影响。为测定细胞内吞作用的变化，采用膜选择性荧光染料FM4-64对HT22细胞在两种太赫兹辐射条件下进行染色，分别为预照射细胞后再进行FM4-64染色和同时照射和FM4-64染色细胞。结果表明，3.1 THz辐照可改变HT22细胞的内吞作用，在细胞处于静息状态时可增强细胞内吞作用，而在细胞处于激发态时则可抑制细胞内吞作用。最后，在GTPase动力蛋白抑制剂dynasore的存在下，两种生理状态下，太z辐射均显著增加了FM4-64的荧光强度。这些发现表明，太赫兹辐射可以正或负改变神经元细胞的内吞过程，提示其有可能影响物质交换和信号传递。(C) 2021美国光学学会
Although more and more attention has been paid to the biological effects of electromagnetic radiation in the terahertz (THz) frequencies range with the rapid development of THz technology, the effects of THz irradiation on many biological processes remain unknown. In this study, we explored the influence of THz irradiation at the frequency of 3.1 THz on the endocytosis of neuronal cells. To determine the changes of endocytosis, a membrane-selective fluorescent dye FM4-64 was used to stain HT22 cells under two kinds of THz irradiation conditions including pre-irradiating cells followed by FM4-64 staining and simultaneously irradiating and FM4-64 dyeing cells. The results showed that 3.1 THz irradiation could alter the endocytosis of HT22 cells via enhancing this process when cells are in a resting state, and, to the contrary, inhibiting this process when cells were in an excited state. Finally, in the presence of dynasore, an inhibitor of GTPase dynamin, THz irradiation dramatically increased the FM4-64 fluorescence intensity comparing to the unirradiated group in both physiological states. These findings demonstrated that THz irradiation could alter the endocytic process of neuronal cells in either a positive or negative way, suggesting that it has the possibility of affecting the substance exchange and signal transmission. (C) 2021 Optical Society of America