The Saccharomyces cerevisiae RanGTP-binding protein msn5p is involved in different signal transduction pathways.
The Saccharomyces cerevisiae RanGTP-binding protein msn5p is involved in different signal transduction pathways.
复制标题
酿酒酵母 RanGTP 结合蛋白 msn5p 参与不同的信号转导途径。
DOI：
10.1093/genetics/153.3.1219
复制
发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Estruch,F
中科院分区：
文献类型：
--
作者：
Alepuz,PM;Matheos,D;Cunningham,KW;Estruch,F
在真核生物中，细胞外信号对转录的控制涉及信号转导途径的至少一种成分易位至细胞核。通过核膜的运输需要与小 GTPase Ran 相互作用的输入或输出受体的活性。我们克隆了酿酒酵母的 MSN5 基因，推测该基因编码这些受体之一。 Msn5p 属于具有保守 N 末端序列的蛋白质家族，该序列充当 RanGTP 结合域。这里提供的结果提供了支持 Msn5p 参与几种不同信号转导途径的遗传数据。所有这些途径都包括基因表达的变化，并且其中一些途径已经描述了响应外部条件而调节成分的核细胞质重新分布。我们按照两种不同的策略克隆了 MSN5。 Msn5p 本质上位于细胞核中。 msn5 突变体的表型分析表明，该蛋白参与分解代谢物抑制、钙信号传导、交配和细胞增殖等过程，并参与先前表征的磷酸盐利用。因此，Msn5p 可能是参与不同信号传导途径的几种蛋白质的受体。
In eukaryotes, control of transcription by extracellular signals involves the translocation to the nucleus of at least one component of the signal transduction pathway. Transport through the nuclear envelope requires the activity of an import or export receptor that interacts with the small GTPase Ran. We have cloned the MSN5 gene of the yeast Saccharomyces cerevisiae that is postulated to encode one of these receptors. Msn5p belongs to a family of proteins with a conserved N-terminal sequence that acts as a RanGTP-binding domain. The results presented here provide genetic data supporting Msn5p involvement in several different signal transduction pathways. All of these pathways include changes in gene expression, and regulated nucleocytoplasmic redistribution of a component in response to external conditions has already been described in some of them. We have cloned MSN5 following two different strategies. Msn5p was constitutively localized in the nucleus. Phenotypic analysis of the msn5 mutant demonstrated that this protein participates in processes such as catabolite repression, calcium signaling, mating, and cell proliferation, as well as being involved in previously characterized phosphate utilization. Therefore, Msn5p could be a receptor for several proteins involved in different signaling pathways.