The photochemistry of the light-, oxygen-, and voltage-sensitive domains in the algal blue light receptor phot
The photochemistry of the light-, oxygen-, and voltage-sensitive domains in the algal blue light receptor phot
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DOI：
10.1002/bip.20510
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发表时间：
2006-07-01
期刊：
影响因子：
2.9
通讯作者：
Heberle, Joachim
中科院分区：
文献类型：
--
作者：
Kottke, Tilman;Hegemann, Peter;Heberle, Joachim
光蛋白是植物和藻类中的蓝光光感受器，主要调节光运动反应。它们包含两个光、氧和电压敏感 (LOV) 结构域和一个丝氨酸/苏氨酸激酶结构域。两个 LOV 结构域均非共价结合黄素单核苷酸 (FMN) 作为发色团。在蓝光照射下，LOV 结构域经历光循环，短暂地形成 FMN 部分与附近半胱氨酸残基的共价加合物。光感受器中存在两个光敏域引发了有关 LOV1 和 LOV2 之间性质和功能差异的问题。作为模型系统，我们对绿藻莱茵衣藻照片中 LOV1 和 LOV2 域的光循环进行了单独和串联结构的详细研究。在这里，我们概述了域的个体行为及其交互的结果。此外，还介绍了目前对 LOV1 在照片中的作用的理解现状。 (c) 2006 年 Wiley 期刊公司
Phot proteins are blue light photoreceptors in plants and algae that mainly regulate photomovement responses. They contain two light-, oxygen-, and voltage-sensitive (LOV) domains and a serine/threonine kinase domain. Both LOV domains noncovalently bind a flavin mononucleotide (FMN) as chromophore. Upon blue light illumination, the LOV domains undergo a photocycle, transiently forming a covalent adduct of the FMN moiety with a nearby cysteine residue. The presence of two light-sensitive domains in the photoreceptor raises the question about the differences in properties and function between LOV1 and LOV2. As a model system, the photocycles of the LOV1 and LOV2 domains from phot of the green alga Chlamydomonas reinhardtii have been studied in detail, both separately and in a tandem construct. Here we give an overview about the results on the individual behavior of the domains and their interaction. Furthermore, the current status in the understanding of the role of LOV1 in phot in general is presented. (c) 2006 Wiley Periodicals, Inc.