Membrane Defects Accelerate Outer Membrane β-Barrel Protein Folding
Membrane Defects Accelerate Outer Membrane β-Barrel Protein Folding
复制标题
DOI：
10.1021/bi501443p
复制
发表时间：
2015-01-20
期刊：
影响因子：
2.9
通讯作者：
Fleming, Karen G.
中科院分区：
文献类型：
--
作者：
Danoff, Emily J.;Fleming, Karen G.
外膜β-桶蛋白自发折叠成脂质双层，其折叠速率受到膜的物理性质的强烈影响。我们表明，当双层处于相变温度时，折叠会加速，因为脂质相域的共存和域边界存在高度缺陷。这些结果与先前观察到的更快折叠成薄且高度弯曲的膜一致，其也包含更高的缺陷发生率。β-桶折叠中缺陷的重要性提供了对内在折叠过程和生物组装途径的深入了解。
Outer membrane beta-barrel proteins spontaneously fold into lipid bilayers with rates of folding that are strongly influenced by the physical properties of the membrane. We show that folding is accelerated when the bilayer is at the phase transition temperature, because of the coexistence of lipid phase domains and the high degree of defects present at domain boundaries. These results are consistent with previous observations of faster folding into thin and highly curved membranes, which also contain a higher prevalence of defects. The importance of defects in beta-barrel folding provides insight into the intrinsic folding process and the biological assembly pathway.