Plasmacytoid dendritic cell-induced migration and activation of NK cells in vivo
Plasmacytoid dendritic cell-induced migration and activation of NK cells in vivo
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DOI：
10.1002/eji.200940098
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发表时间：
2010-08-01
影响因子：
5.4
通讯作者：
Chambers, Benedict J.
中科院分区：
文献类型：
--
作者：
Persson, Catrine M.;Chambers, Benedict J.
NK细胞是先天免疫系统的细胞毒性细胞。它们被发现在抵抗感染和一些肿瘤方面至关重要。最近的研究表明，NK细胞需要来自辅助细胞的信号来诱导它们在感染或肿瘤生长部位的募集和激活。在这项研究中，我们研究了浆细胞样DC（pDC）是否可以在体内招募和激活NK细胞。当将CpG刺激的pDC腹膜内注射至C57 BL/6小鼠时，它们有效地募集NK细胞，这一过程依赖于NK细胞CXCR 3和CD 62 L，部分依赖于CCR 5。从用TLR 7/8或TLR 9刺激的pDC接种的小鼠的腹膜分离的NK细胞比从用对照pDC接种的小鼠回收的NK细胞表现出更大的针对YAC-1肿瘤细胞的细胞毒性。目前的结果进行了讨论，在pDC诱导的NK细胞迁移和激活在体内。
NK cells are cytotoxic cells of the innate immune system. They have been found to be critical in the defense against infections and also against some tumors. Recent studies have shown that NK cells require signals from accessory cells to induce their recruitment and activation at the site of infection or tumor growth. In this study, we examined whether plasmacytoid DC (pDC) could recruit and activate NK cells in vivo. When CpG-stimulated pDC were injected i.p. to C57BL/6 mice, they efficiently recruited NK cells, a process that was dependent on NK cell CXCR3 and CD62L and in part on CCR5. NK cells isolated from the peritoneum of mice inoculated with TLR7/8 or TLR9-stimulated pDC exhibited greater cytotoxicity against YAC-1 tumor cells than NK cells recovered from mice inoculated with control pDC. The present results are discussed in relation to pDC-induced NK cell migration and activation in vivo.