Present status of large-scale culture methods of pluripotent stem cell : Differentiation of human iPS cells with dialysis suspension culture
Present status of large-scale culture methods of pluripotent stem cell : Differentiation of human iPS cells with dialysis suspension culture
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多能干细胞大规模培养方法的现状：透析悬浮培养人iPS细胞的分化
DOI：
10.11378/organbio.26.175
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
酒井 康行
中科院分区：
文献类型：
--
作者：
チェ ヒョンジン;篠原 満利恵;酒井 康行
多能干细胞（Pluripotent stem cells，PSC）具有高度的自我更新能力和多能性，有望成为再生医学和细胞药物筛选领域的重要细胞来源。为了实现这些应用，非常需要大规模和高效的细胞生产方法。本文简要介绍了几种典型的悬浮培养方法及其优缺点。此外，我们解释了我们最新的试验，关于胰腺β细胞分化的人诱导多能干细胞（hiPSC）使用悬浮培养系统与透析操作。
Pluripotent stem cells (PSCs) are expected to be a promising cell source in the field of regenerative medicine and cell-based drug screening because of their high self-renewal capability while preserving pluripotency. To realize these applications, large-scale and efficient cell production methodologies are highly required. In this short review, we introduced typical suspension culture methods with their advantages and disadvantages. In addition, we explained our latest trials concerning pancreatic β cells differentiation of human induced pluripotent stem cells (hiPSCs) using suspension culture system with dialysis operations.