Advanced molecular immunoassay system for immunobiotic lactic acid bacteria using a transfectant of Toll-like receptor 2
Advanced molecular immunoassay system for immunobiotic lactic acid bacteria using a transfectant of Toll-like receptor 2
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DOI：
10.1111/j.1740-0929.2007.00425.x
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发表时间：
2007-04-01
影响因子：
2
通讯作者：
Kitazawa, Haruki
中科院分区：
文献类型：
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作者：
Tohno, Masanori;Shimosato, Takeshi;Kitazawa, Haruki
Toll样受体2（TLR 2）是多种微生物组分的受体，并且它还介导与先天免疫系统相关的细胞中的激活信号。为了研究不同乳酸菌（LAB）通过TLR 2的精确分子免疫调节作用，利用人胚肾（HEK）293细胞构建了表达猪TLR 2（sTLR 2）的转染子。研究表明，完整的免疫生物活性LAB可通过TLR 2诱导免疫应答，不同菌株的不同核因子-κ B（NF-κ B）活性可通过表达sTLR 2的HEK 293细胞准确检测。此外，经由TLR 2的NF-κ B的细胞活化反映在LAB的增强的结合和摄取中。表达sTLR 2的HEK 293细胞也可用于表征通过免疫生物LAB刺激的I型辅助T（Th 1）和II型辅助T（Th 2）细胞因子的表达模式。这些结果表明，sTLR 2表达HEK 293细胞可能是有用的，在某些分子免疫测定系统生产新的生理功能的食品与肠道免疫调节能力，如维持Th 1/Th 2极化。
Toll-like receptor 2 (TLR2) is a receptor for a variety of microbial components, and it also mediates activation signals in the cell relating to the innate immune system. In order to evaluate the precise molecular immunoregulation by various strains of lactic acid bacteria (LAB) via TLR2, the swine TLR2 (sTLR2)-expressing transfectant was constructed using human embryonic kidney (HEK) 293 cells. It is demonstrated that intact immunobiotic LAB can induce immune responses through TLR2, and that different nuclear factor-kappa B (NF-kappa B) activities of various strains can be accurately detected by sTLR2-expressing HEK293 cells. Furthermore, cellular activation of NF-kappa B via TLR2 is reflected in enhanced binding and uptake of LAB. The sTLR2-expressing HEK293 cells were also useful for characterizing the expression pattern of type I helper T (Th1) and type II helper T (Th2) cytokines by the stimulation of immunobiotic LAB. These results suggest that sTLR2-expressing HEK293 cells may be useful in certain molecular immunoassay systems for producing new physiologically functional foods with intestinal immunomodulatory abilities, such as the maintenance of Th1/Th2 polarization.