Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative
Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative
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从芽霉素纯化衍生物中制备性等速电泳分离皮试抗原
DOI：
10.1128/iai.13.3.758-762.1976
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发表时间：
1976
影响因子：
3.1
通讯作者：
R. Sprouse
中科院分区：
文献类型：
--
作者：
M. Lancaster;R. Sprouse
本研究探讨了芽生霉素纯衍生物的预电泳分离所需的理论和实际参数。它产生了一种相对简单的两步程序来制备毫克数量的芽生霉素抗原，该抗原在实验感染的豚鼠中表现出敏感性和特异性。分析9个等速电泳组分的皮试灵敏度和特异性提供了一些见解，普遍接受的不可靠性的芽生霉素时，用于免疫学评价。
This investigation examined the theoretical and practical parameters requisite to preparatory istachophoretic separation of blastomycin purified derivative. It resulted in a relatively simple, two-step procedure for preparation of blastomycin antigens in milligram quantitites that exhibited sensitivity and specificity in experimentally infected guinea pigs. Analysis of the nine isotachophoretic fractions for skin test sensitivity and specificity provided some insight into the generally accepted unreliability of blastomycin when used for immunological evaluation.