HIGH-TEMPERATURE INDUCTION OF A STRINGENT RESPONSE IN THE DNAK(TS) AND DNAJ(TS) MUTANTS OF ESCHERICHIA-COLI
HIGH-TEMPERATURE INDUCTION OF A STRINGENT RESPONSE IN THE DNAK(TS) AND DNAJ(TS) MUTANTS OF ESCHERICHIA-COLI
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DOI：
10.1093/oxfordjournals.jbchem.a135648
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发表时间：
1986-06-01
影响因子：
2.7
通讯作者：
WADA, M
中科院分区：
文献类型：
--
作者：
ITIKAWA, H;FUJITA, H;WADA, M
检测了大肠杆菌dnaK（Ts）和dnaJ（Ts）突变体中ppGpp的细胞浓度，因为这些突变体的热敏性RNA合成因relA基因中的额外突变而松弛。结果表明，ppGpp在温度升高后在dnaK（Ts）和dnaJ（Ts）突变体中广泛积累，分别达到5 mM和0.5 mM的水平。这种不寻常的积累ppGpp抑制relA1突变，这意味着它的结果从诱导严格的反应，在这些突变体在非允许的温度。
The cellular concentrations of ppGpp in the dnaK(Ts) and dnaJ(Ts) mutants of Escherichia coli were examined, since the thermosensitive RNA synthesis of these mutants is relaxed by an additional mutation in the relA gene. The results showed that ppGpp accumulated extensively in the dnaK(Ts) and dnaJ(Ts) mutants after a temperature shift up, reaching levels of 5 mM and 0.5 mM, respectively. This unusual accumulation of ppGpp was suppressed by the relA1 mutation, implying that it results from induction of a stringent response in these mutants at a nonpermissive temperature.