Automated Purification of DNA Origami with SPRI Beads
Automated Purification of DNA Origami with SPRI Beads
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DOI：
10.1002/smll.202308776
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发表时间：
2023-12-06
期刊：
影响因子：
13.3
通讯作者：
Walti，Christoph
中科院分区：
文献类型：
--
作者：
Chau，Chalmers;Mohanan，Gayathri;Walti，Christoph
DNA折纸合成是一种成熟的技术，具有广泛的应用。在大多数情况下，合成的折纸必须经过纯化，以去除多余的材料，如DNA寡核苷酸和其他功能分子。虽然常规使用几种纯化技术，但都有局限性，并且不能与机器人系统集成。在这里，使用固相可逆固定（SPRI）珠作为一种可扩展的，高通量的，和自动化的方法来纯化DNA折纸被证明。这种方法不仅可以去除未反应的寡核苷酸和生物分子，其产率与现有方法相当，同时保持折纸的高度结构完整性，而且还可以集成到自动化工作流程中，以同时纯化大量样品。可以预见，SPRI珠纯化方法将提高DNA纳米结构合成的可扩展性，用于研究和商业应用。
DNA origami synthesis is a well‐established technique with wide‐ranging applications. In most cases, the synthesized origami must be purified to remove excess materials such as DNA oligos and other functional molecules. While several purification techniques are routinely used, all have limitations, and cannot be integrated with robotic systems. Here the use of solid‐phase reversible immobilization (SPRI) beads as a scalable, high‐throughput, and automatable method to purify DNA origami is demonstrated. Not only can this method remove unreacted oligos and biomolecules with yields comparable to existing methods while maintaining the high structural integrity of the origami, but it can also be integrated into an automated workflow to purify simultaneously large numbers and quantities of samples. It is envisioned that the SPRI beads purification method will improve the scalability of DNA nanostructures synthesis both for research and commercial applications.