Separation and determination of n-alkylamines and histamine by capillary zone electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent.
Separation and determination of n-alkylamines and histamine by capillary zone electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent.
复制标题
使用 5- 磺酸水杨醛作为衍生剂，通过毛细管区带电泳分离和测定正烷基胺和组胺。
DOI：
10.1016/s0021-9673(01)01280-8
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发表时间：
2001
影响因子：
4.1
通讯作者：
S. Motomizu
中科院分区：
文献类型：
--
作者：
R. Driouich;T. Takayanagi;M. Oshima;S. Motomizu
研究了水杨醛-5-磺酸钠 (SAS) 作为衍生试剂用于通过毛细管区带电泳 (CZE) 分离和测定伯胺。在测定之前，用 SAS 将胺衍生为相应的席夫碱。详细研究了席夫碱形成反应和 CZE 分析的最佳条件。在 40°C 的 40%(v/v) 乙醇溶液中，希夫碱在 9 分钟内几乎完全形成。发现含有 40%（v/v）乙醇和 20 mM 磷酸盐缓冲液（pH 7.8）的迁移溶液对于希夫碱的稳定性是优选的。在最佳条件下用SAS对8种正烷基胺进行衍生化，并通过CZE分析成功分离衍生物。所提出的方法可以同时、灵敏且足够精确地测定烷基链长度为 3 至 12 个亚甲基的正烷基胺。 SAS 衍生化过程已成功应用于极低水平组胺的检测。检测限为2.5·10−6M，与未衍生化的CZE分析相比提高了8倍左右。
Sodium salicylaldehyde-5-sulfonate (SAS) was investigated as a derivatizing reagent for the separation and determination of primary amines by capillary zone electrophoresis (CZE). The amines were derivatized with SAS to the corresponding Schiff bases before their determination. Optimal conditions for the formation reactions of the Schiff bases and the CZE analysis were investigated in details. The Schiff bases were formed almost completely within 9 min in 40%(v/v) ethanol solution at 40°C. A migrating solution containing 40%(v/v) ethanol and 20 mM phosphate buffer (pH 7.8) was found to be preferable for the stability of the Schiff bases. Eight kinds of n-alkylamines were derivatized with SAS under the optimal conditions and the derivatives were successfully separated by a CZE analysis. The proposed method allows simultaneous, sensitive and sufficiently precise determination of the n-alkylamines with the alkyl chain length from 3 to 12 of methylene groups. The derivatization process with SAS was successfully applied to the detection of histamine at a very low level. The detection limit was 2.5·10−6M, and it was improved in the order of 8 times compared with the CZE analysis without derivatization.