Resident memory T cells: Possible players in periodontal disease recurrence
Resident memory T cells: Possible players in periodontal disease recurrence
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DOI：
10.1111/jre.12709
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发表时间：
2019-11-02
影响因子：
3.5
通讯作者：
Aimetti, Mario
中科院分区：
文献类型：
--
作者：
Deli, Federico;Romano, Federica;Aimetti, Mario
背景/目标组织驻留记忆 T 细胞 (Trm) 代表长寿命记忆 T 细胞的一个新子集，它们在先前的细菌或病毒感染后保留在屏障组织中，以支持早期/立即防御机制，提供针对病原体攻击的位点特异性保护。由于人类牙龈中 Trm 细胞的数据刚刚出现，本研究的目的是探讨它们在上皮和结缔牙周组织中的存在和分布与微生物暴露和牙周损伤的关系。材料和方法 牙周炎组织标本是从 20 名全身性慢性牙周炎患者骨切除手术时采集的。作为对照，在粘龈手术期间从 18 名牙周健康患者的原发瓣和腭移植物中各采集了 18 个健康组织样本。由于 CD69 和 CD103 是与组织驻留相关的表型标记物，因此在表达最高的区域对每个高倍视野的上皮内和基质 CD103+ 和 CD69+ 细胞进行计数。对 CD3 和 CD69 进行双重免疫组织化学分析以鉴定 T 细胞。结果CD69+和CD103+细胞呈淋巴细胞形态，CD69和CD3双重染色证实了这些细胞的T细胞表型。与健康对照相比，牙周炎患者的上皮和结缔组织中 CD103 和 CD69 的表达显着增强 (P < .001)。在牙周炎患者的组织标本中观察到 PD 与 CD103 和 CD69 上皮表达之间存在显着正相关性 (P < .001)。结论 在本研究的范围内，这些结果表明牙周炎病变中 Trm 细胞较高。他们可以协调宿主对微生物挑战的反应，从而导致牙周病更快地重新激活。
Background/objectives Tissue-resident memory T cells (Trm) represent a new subset of long-lived memory T cells that remain in barrier tissues after previous bacterial or viral infection to support early/immediate defense mechanisms, providing site-specific protection from pathogen challenge. As data on Trm cells in human gingiva are just emerging, the aim of the present study was to explore their presence and distribution in epithelial and connective periodontal tissues in relation to microbial exposure and periodontal damage. Material and methods Periodontitis tissue specimens were collected from 20 generalized chronic periodontitis patients at the time of osseous resective surgery. As a control, 18 healthy tissue specimens were harvested each from both the primary flap and the palatal graft in 18 periodontally healthy patients during mucogingival surgeries. As CD69 and CD103 are phenotypic markers associated with tissue residence, intraepithelial and stromal CD103+ and CD69+ cells per high-power field were counted in areas with highest expression. Double immunohistochemistry for CD3 and CD69 was performed to identify T cells. Results CD69 +and CD103+ cells showed a lymphocytic morphology, and double CD69 and CD3 staining confirmed the T cell phenotype of these cells. CD103 and CD69 expression was significantly enhanced in epithelial and connective tissues from patients with periodontitis compared with healthy controls (P < .001). Significant positive correlation between PD and both CD103 and CD69 epithelial expression was observed in tissue specimens from periodontitis patients (P < .001). Conclusion Within the limits of the present study, these results indicate that Trm cells are higher in periodontitis lesions. They could orchestrate the host response to microbial challenge, leading to a faster reactivation of periodontal disease.