Development and application of a focused ultrasoft x-ray probe for radiobiological applications
Development and application of a focused ultrasoft x-ray probe for radiobiological applications
复制标题
用于放射生物学应用的聚焦超软X射线探头的开发和应用
DOI：
10.1117/12.450232
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发表时间：
2001
影响因子：
1.7
通讯作者：
B. Michael
中科院分区：
文献类型：
--
作者：
M. Folkard;G. Schettino;B. Vojnovic;A. Michette;C. David;S. Pfauntsch;K. Prise;B. Michael
细胞微辐射现在被视为了解辐射如何与活细胞和组织相互作用的有效方法。这项技术的优势在于它能够将精确剂量的辐射传递到体外选定的单个细胞或细胞内预先选定的目标。我们最近开发了一种聚焦软X射线微探针，用于靶向单个细胞。使用聚焦X射线进行此类研究是独一无二的，并且正在应用于许多新颖的实验中。一个重要的应用是研究所谓的旁观者效应，即未受辐射的细胞对附近受辐射细胞的信号做出反应。它也被用作亚细胞探针，以比较细胞核与细胞质靶向的效果。我们的设备使用0.4-0.8 mm直径的波带片将软X射线聚焦成亚微米光束。然后使用快速自动化细胞寻找和比对程序瞄准选定的亚细胞目标。波带片成像碳或铝的特征K X射线，通过将5-10 keV的电子束聚焦到适当的目标上产生。目前的安排将提供约10000光子/秒的焦点（足以照射几十个细胞每分钟）。
Cellular micro-irradiation is now seen as a potent method for understanding how radiations interact with living cells and tissues. The strength of this technique lies in its ability to deliver precise doses of radiation to selected individual cells in vitro, or to pre-selected targets within cells. We have recently developed a focused soft X-ray microprobe for targeting individual cells. The use of focused X-rays for this type of study is unique, and is being applied in a number of novel experiments. One important application is to study the so-called bystander effect where un-irradiated cells are seen to respond to signals from nearby irradiated cells. It is also being used as a sub-cellular probe to compare the effects of nuclear versus cytoplasmic targeting. Our facility uses a 0.4-0.8 mm diameter zone plate to focus soft X-rays to a sub-micron beam. This is then aimed at selected sub-cellular targets using rapid automated cell finding and alignment procedures. The zone plate images characteristic-K X-rays of carbon or aluminium, generated by focusing a beam of 5-10 keV electrons on to the appropriate target. The current arrangement will deliver about 10000 photons/sec to the focus (sufficient to irradiate several tens of cells per minute).
DOI：
10.1073/pnas.94.8.3765
发表时间：
1997-04-15
影响因子：
11.1
作者：
Hei, TK;Wu, LJ;RandersPehrson, G
通讯作者：
RandersPehrson, G
DOI：
10.1073/pnas.030420797
发表时间：
2000-02-29
影响因子：
11.1
作者：
Zhou, HN;Randers-Pehrson, G;Hei, TK
通讯作者：
Hei, TK