INHIBITORY EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON DNA-SYNTHESIS OF ADULT-RAT HEPATOCYTES IN PRIMARY CULTURE
INHIBITORY EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON DNA-SYNTHESIS OF ADULT-RAT HEPATOCYTES IN PRIMARY CULTURE
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DOI：
10.1016/0006-291x(85)91241-0
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发表时间：
1985-12-31
影响因子：
3.1
通讯作者：
ICHIHARA, A
中科院分区：
文献类型：
--
作者：
NAKAMURA, T;TOMITA, Y;ICHIHARA, A
一种转化生长因子-β。（TGF-β）在血小板中发现的1，2-二氯-2-苯并咪唑（1，2-dihydroxyphenyl）可强烈抑制胰岛素加EGF或大鼠血小板肝细胞生长因子（HGF）刺激的原代培养的成年大鼠肝细胞DNA合成，但对细胞分泌蛋白和细胞内蛋白的合成无明显影响。TGF-.beta.通过相差显微镜检查细胞形态判断，没有细胞毒性作用。TGF-β对DNA合成的抑制与标记指数的显著降低相关。TGF-.beta.不抑制肝癌细胞系的生长。这些发现表明TGF-β.是成年大鼠肝细胞的强抑制剂，可阻断其从G1期向S期的转变。TGF-β的生理作用在肝再生过程中抑制成年肝细胞的生长进行了讨论。
A transforming growth factor-.beta.(TGF-.beta.) found in platelets strongly inhibited DNA synthesis of adult rat hepatocytes in primary culture stimulated by insulin plus EGF or by hepatocyte growth factor (HGF) from rat platelets, but not the syntheses of secretory and intracellular proteins by the cells. TGF-.beta. had no cytotoxic effect, as judged by phase-contrast microscopic examination of the cell morphology. The inhibition of DNA synthesis by TGF-.beta. was correlated with marked decrease in the labeling index. TGF-.beta. did not inhibit growth of hepatoma cell line. These findings indicate that TGF-.beta. is a strong inhibitor of adult rat hepatocytes and may block their shift from the G1 phase to the S phase. The physiological role of TGF-.beta. in inhibiting growth of adult hepatocytes during liver regeneration is discussed.