Accelerated biodegradation of poly(vinyl alcohol) by glycosidations of the hydroxyl groups or addition of sugars
Accelerated biodegradation of poly(vinyl alcohol) by glycosidations of the hydroxyl groups or addition of sugars
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DOI：
10.1021/bm034527q
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发表时间：
2004-05-01
影响因子：
6.2
通讯作者：
Kinoshita, T
中科院分区：
文献类型：
--
作者：
Takasu, A;Takada, M;Kinoshita, T
研究了N-乙酰-D-氨基葡萄糖（GlcNAc）-（1）和壳聚糖取代的聚乙烯醇（PVA）在土壤悬浮液（pH6.5）中于25 ℃下40天的生物降解性。在降解条件下，取代度为0.2-0.3（DP = 430-480）的I的生化需氧量高于PVA。尺寸排阻色谱，H-1 NMR，和傅立叶变换红外测量回收的样品表明，PVA主链的生物降解加速PVA中的羟基的部分糖苷化。在PVA/GlcNAc（50：50，w/w）混合物中观察到类似的加速。与糖苷化聚合物的降解相关的微生物在包括土壤悬浮液和作为碳源的聚合物的培养基中生长。聚丙烯酰胺凝胶电泳（SDS-PAGE）和红外光谱测定表明，无细胞提取物来自GIcNAc取代的PVA是不同的PVA/GIcNAc混合物。结果表明，在GlcNAc-取代的PVA中的PVA主链被不同的微生物裂解或通过与混合物中不同的机制裂解。壳聚糖取代的PVA 2表现出更强的加速作用，表明糖长度影响降解性。
Biodegradabilities of N-acetyl-D-glucosamine (GlcNAc)-(1) and chitobiose-substituted (2) poly(vinyl alcohol)s (PVA)s in a soil Suspension (pH 6.5) were investigated at 25 degreesC for 40 days. Biochemical oxygen demand of I with a degree of substitution of 0.2-0.3 (DP = 430-480) was higher than that of PVA under the degradation condition. Size exclusion chromatography, H-1 NMR, and Fourier-transform infrared measurements of the recovered sample indicated that biodegradation of the PVA main chain was accelerated by partial glycosidation of hydroxyl groups in PVA. Similar acceleration was observed in a PVA/GlcNAc (50:50, w/w) mixture. Microbes which relate with degradation of the glycosidated polymers were grown in a culture medium including the soil suspension and the polymer as the carbon source. Polyacrylamide gel electrophoresis (SDS-PAGE) and IR measurements indicated that a cell-free extract derived from GlcNAc-substituted PVA was different from that in the PVA/GlcNAc mixture. The results suggested that the PVA main chain in GlcNAc-substitLited PVA was cleaved by a different microorganism or via a mechanism different from that in the mixture. Chitobiose-substituted PVA 2 showed more enhanced acceleration, indicating that the sugar length influenced the degradability.