Preparation and characterization of tentacle-type polymer stationary phase modified with graphene oxide for open-tubular capillary electrochromatography
Preparation and characterization of tentacle-type polymer stationary phase modified with graphene oxide for open-tubular capillary electrochromatography
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DOI：
10.1016/j.chroma.2015.04.039
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发表时间：
2015-06-26
影响因子：
4.1
通讯作者：
Ji, Yibing
中科院分区：
文献类型：
--
作者：
Gao, Xing;Mo, Rongzhen;Ji, Yibing
本研究制备了一种新型无机纳米材料修饰的有机聚合物涂层固定相，用于开管毛细管电色谱（OT-CEC）。该毛细管是通过将氧化石墨烯（GO）共价连接到用氢氧化铵衍生的触角型聚合物链接枝毛细管上而开发的。扫描电子显微镜（SEM）和拉曼光谱提供的证据表明，触手型聚合物和GO被涂覆到毛细管内壁。该柱在pH值为4.0 ~ 9.0的电解质溶液中能产生稳定的电渗流，对中性小分子、氨基酸和麻黄碱-伪麻黄碱异构体具有良好的分离性能，分离度大于2.30，柱效达17万板/m。GO-GMA@毛细管柱的重复性令人满意，日内和日间运行的相对标准偏差小于3.27%，柱间运行的相对标准偏差小于7.49%。此外，还比较了GO-聚合物修饰毛细管、聚合物涂层毛细管和GO涂层毛细管的分离性能。(C)2015 Elsevier B. V.版权所有。
In this research, a novel organic polymer coated stationary phase modified with inorganic nanomaterial was prepared for open-tubular capillary electrochromatography (OT-CEC). The capillary was developed by covalent attaching graphene oxide (GO) onto the tentacle-type polymer chains grafted capillary which was derivatized with ammonium hydroxide. Scanning electron microscopy (SEM) and Raman spectroscopy provided evidences that the tentacle-type polymer and GO were coated to the capillary inner wall. This novel GO-polymer modified capillary column generated a strong and stable electroosmotic flow (EOF) from anode to cathode with the running electrolytes of pH ranging from 4.0 to 9.0, and showed excellent separation ability toward neutral small molecules, amino acids and ephedrine-pseudoephedrine isomers with resolution higher than 2.30 and column efficiency up to over 170,000 plates/m. The repeatabilities of the GO-GMA@capillary were satisfying with relative standard deviations for intra-day and inter-day runs less than 3.27%, and column-to-column runs less than 7.49%. Additionally, the separation performance of GO-polymer modified capillary was compared with that of the polymer coated capillary and GO coated capillary. (C) 2015 Elsevier B.V. All rights reserved.